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人轮状病毒分离株CY2017的分离与特性

Isolation and characteristics of the human rotavirus isolate CY2017.

作者信息

Song Yan, Liu Wei

机构信息

Affiliated Chuiyangliu Hospital of Tsinghua University, Beijing, 100022 China.

出版信息

Virusdisease. 2020 Sep;31(3):257-261. doi: 10.1007/s13337-020-00607-w. Epub 2020 Jun 4.

DOI:10.1007/s13337-020-00607-w
PMID:32904816
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7458959/
Abstract

Our study aimed to determine whether a virus from fecal samples is human rotavirus (HRV), the main pathogen that causes autumn diarrhea. Specimens were obtained from the feces from children with autumn diarrhea treated at Chuiyangliu Hospital and used to infect MA104 cells, subcultured and observed by electron microscopy. RNA was extracted, cDNA was synthesized by reverse transcription, and plaque formation and hemagglutination were assessed. The cytopathic effect (CPE) were associated with the fourth passage in subculture. CPE extracts were examined by electron microscopy, which allowed us to observe the shape of HRV particles. Amplification of the VP4 gene from HRV was used to identify the viruses as group A rotavirus. The virus causes red blood cell aggregation. The virus isolate was designated as HRV CY2017.

摘要

我们的研究旨在确定粪便样本中的病毒是否为人轮状病毒（HRV），这是导致秋季腹泻的主要病原体。样本取自垂杨柳医院治疗的秋季腹泻患儿的粪便，用于感染MA104细胞，传代培养并通过电子显微镜观察。提取RNA，通过逆转录合成cDNA，并评估噬斑形成和血凝反应。细胞病变效应（CPE）与传代培养的第四次传代有关。通过电子显微镜检查CPE提取物，使我们能够观察到HRV颗粒的形状。通过扩增HRV的VP4基因来鉴定病毒为A组轮状病毒。该病毒导致红细胞聚集。该病毒分离株被命名为HRV CY2017。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b04/7458959/b490d97a145c/13337_2020_607_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b04/7458959/57ea13c281eb/13337_2020_607_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b04/7458959/0cd4d75aa6cc/13337_2020_607_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b04/7458959/76641b660572/13337_2020_607_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b04/7458959/b490d97a145c/13337_2020_607_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b04/7458959/57ea13c281eb/13337_2020_607_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b04/7458959/0cd4d75aa6cc/13337_2020_607_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b04/7458959/76641b660572/13337_2020_607_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b04/7458959/b490d97a145c/13337_2020_607_Fig4_HTML.jpg

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Human VP8* mAbs neutralize rotavirus selectively in human intestinal epithelial cells.人源 VP8* 单抗在人肠道上皮细胞中选择性中和轮状病毒。
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