Key Laboratory of Public Health Safety of Hebei Province, College of Pharmacy, Hebei University, Baoding, 071002, China, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China.
Key Laboratory of Public Health Safety of Hebei Province, College of Pharmacy, Hebei University, Baoding, 071002, China, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Nov 30;1159:122343. doi: 10.1016/j.jchromb.2020.122343. Epub 2020 Aug 28.
In this study, modified UiO-66-NH and N-methylolacrylamide (NMA) were used as common monomers to prepare a metal organic framework (MOF)-based composite monolith through in-situ polymerization, which was used as a new adsorbent to purify and enrich aristolochic acid-I (AA-I) in medicinal plants. The MOF-based composite monolithic column was characterized by nitrogen adsorption-desorption isotherm, mercury intrusion porosimetry and scanning electron microscopy (SEM). The adsorption ability of MOF-based composite monolith for AA-I was compared with that of the polymer monolith without MOF added. The results proved that the addition of UiO-66-NH can increase both the specific surface area and the permeability of the monolith. Moreover, the adsorption amount of AA-I on the monolith improved. This proposed on-line solid phase extraction (SPE) method showed good linear relationship in the range 0.044 ~ 400 μg/mL with r = 0.9994; the limit of detection (LOD) was 13.08 ng/mL and the limit of quantification (LOQ) was 44.00 ng/mL; the intra-day and inter-day accuracies were less than 0.97%; the inter-column accuracies was less than 6.11%; the recovery was in the range of 91.11%~106.48%. The method was found to be easy, accurate and convenient for on-line enrichment and purification of AA-I in medicinal plants.
在这项研究中，使用改性的 UiO-66-NH 和 N-羟甲基丙烯酰胺（NMA）作为常见单体，通过原位聚合制备了一种金属有机骨架（MOF）基复合整体柱，用作从药用植物中纯化和富集马兜铃酸-I（AA-I）的新型吸附剂。通过氮气吸附-解吸等温线、压汞孔隙率和扫描电子显微镜（SEM）对 MOF 基复合整体柱进行了表征。比较了 MOF 基复合整体柱对 AA-I 的吸附能力与未添加 MOF 的聚合物整体柱的吸附能力。结果证明，UiO-66-NH 的添加可以同时提高整体柱的比表面积和渗透性。此外，AA-I 在整体柱上的吸附量也得到了提高。所提出的在线固相萃取（SPE）方法在 0.044 至 400 μg/mL 范围内具有良好的线性关系，r = 0.9994；检测限（LOD）为 13.08 ng/mL，定量限（LOQ）为 44.00 ng/mL；日内和日间精度均小于 0.97%；柱间精度小于 6.11%；回收率在 91.11%至 106.48%范围内。该方法易于操作、准确且方便，可用于药用植物中 AA-I 的在线富集和纯化。
