Liu Hongnan, Tan Bie, Kong Xiangfeng, Li Jianjun, Li Guangran, He Liuqin, Bai Miaomiao, Yin Yulong
Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, 644 Yuanda 2 Road, Changsha 410125, Hunan, China.
Animals (Basel). 2020 Sep 7;10(9):1590. doi: 10.3390/ani10091590.
This study was conducted to evaluate the effects of dietary insect powder supplementation as a protein source on plasma amino acid profiles, intestinal amino acid transport and sensing in a piglet model. A total of 144 weanling piglets were randomly assigned to four experimental diets for two phases (Days 1-28 and Days 29-56), to assess the effects on amino acid profiles and transportation in the segments of the intestine. The groups were basal diet (control), control diet plus (TM), control diet plus larvae (MDL) and control diet plus (ZM). The plasma free amino acid levels were stable comparable among treatments, except that the lysine level was significantly reduced by dietary MDL and ZM supplementation in the first phase ( < 0.05). In the 1st phase, the sensitivity of intestinal segments to the regulation of the amino acid level by insect powder supplementation follows sequence: colon > ileum > jejunum, while the order switched to jejunum > colon > ileum in the 2nd phase. The relative RNA expressions of mitogen-activated protein 4 kinase 3 (MAP4K3), sodium dependent neutral amino acid transporter2 (SNAT2), the transient receptor potential cation channel subfamily V member 1 (TRPV1) and taste 1 receptor member 1/3 (T1R3) in the segments of the intestine were affected by different dietary insect powder supplementation. G protein-coupled receptor family C group 6 member A (GPRC6A) level in the jejunal and colonic mucosa was upregulated by MDL supplementation ( < 0.05). These results indicated that dietary insects improved the metabolism of the amino acid in the prophase (the 1st phase) through regulating the sensing gene and mTOR signal pathway in intestinal mucosa by targeting different receptors. The finding demonstrates that the insect powder is a potentially promising source for protein deposition.
本研究旨在评估在仔猪模型中添加膳食昆虫粉作为蛋白质来源对血浆氨基酸谱、肠道氨基酸转运和感知的影响。总共144头断奶仔猪被随机分配到四种实验日粮中，进行两个阶段（第1 - 28天和第29 - 56天）的实验，以评估对肠道各段氨基酸谱和转运的影响。分组为基础日粮（对照组）、对照组日粮加 （TM）、对照组日粮加 幼虫（MDL）和对照组日粮加 （ZM）。除第一阶段日粮添加MDL和ZM使赖氨酸水平显著降低外（<0.05），各处理间血浆游离氨基酸水平稳定且相当。在第一阶段，肠道各段对添加昆虫粉调节氨基酸水平的敏感性顺序为：结肠>回肠>空肠，而在第二阶段顺序变为空肠>结肠>回肠。肠道各段中丝裂原活化蛋白4激酶3（MAP4K3）、钠依赖性中性氨基酸转运体2（SNAT2）、瞬时受体电位阳离子通道亚家族V成员1（TRPV1）和味觉1受体成员1/3（T1R3）的相对RNA表达受到不同膳食昆虫粉添加的影响。MDL添加使空肠和结肠黏膜中的G蛋白偶联受体家族C组6成员A（GPRC6A）水平上调（<0.05）。这些结果表明，膳食昆虫通过靶向不同受体调节肠道黏膜中的感知基因和mTOR信号通路，在前期（第一阶段）改善了氨基酸代谢。该发现表明昆虫粉是一种潜在的有前景的蛋白质沉积来源。
