Puppel Kamila, Kalińska Aleksandra, Kot Magdalena, Slósarz Jan, Kunowska-Slósarz Małgorzata, Grodkowski Grzegorz, Kuczyńska Beata, Solarczyk Paweł, Przysucha Tomasz, Gołębiewski Marcin
Institute of Animal Science, Warsaw University of Life Sciences, 02-786 Warsaw, Poland.
Animals (Basel). 2020 Sep 7;10(9):1591. doi: 10.3390/ani10091591.
Mastitis is one of the most common diseases of high-yielding dairy cows, and over 90% of cases are caused by spp., , or spp. Certain groups of proteins are very significant in terms of the cow's antioxidant, bacteriostatic, and germicidal properties: lysozyme (Lz), lactoferrin (Lf), and β-lactoglobulin (BLG). This study aimed to determine the influence of spp., spp., and Enterobacteriaceae on the secretion of bioactive whey proteins and oxidative stress markers. From the herd, 60 multiparous cows with diagnosed mastitis were selected. Samples were taken individually from each quarter and pooled, which gave 60 samples. did not affect the BLG synthesis, whereas lysozyme and lactoferrin responded to a high concentration of these bacterial strains. In the case of spp. infection, the BLG level increased. These strains did not affect the levels of di-malonic aldehyde (MDA), lactoferrin, and lysozyme. In contrast, they were significantly influenced by spp. In summary, the levels of whey proteins and oxidative stress markers changed depending on the bacterial strain inducing inflammation. Lysozyme and lactoferrin may be markers of udder inflammation caused by and spp., whereas β-lactoglobulin may prove useful in diagnosing spp. induced mastitis.
乳腺炎是高产奶牛最常见的疾病之一，超过90%的病例由金黄色葡萄球菌、无乳链球菌或停乳链球菌引起。某些蛋白质组在奶牛的抗氧化、抑菌和杀菌特性方面非常重要：溶菌酶（Lz）、乳铁蛋白（Lf）和β-乳球蛋白（BLG）。本研究旨在确定金黄色葡萄球菌、无乳链球菌和肠杆菌科对生物活性乳清蛋白分泌和氧化应激标志物的影响。从牛群中挑选出60头诊断为乳腺炎的经产奶牛。从每个乳区单独采集样本并合并，共得到60个样本。金黄色葡萄球菌不影响β-乳球蛋白的合成，而溶菌酶和乳铁蛋白对这些细菌菌株的高浓度有反应。在感染无乳链球菌的情况下，β-乳球蛋白水平升高。这些菌株不影响丙二醛（MDA）、乳铁蛋白和溶菌酶的水平。相比之下，它们受停乳链球菌的影响显著。总之，乳清蛋白水平和氧化应激标志物根据引发炎症的细菌菌株而变化。溶菌酶和乳铁蛋白可能是由金黄色葡萄球菌和停乳链球菌引起的乳房炎症的标志物，而β-乳球蛋白可能在诊断无乳链球菌引起的乳腺炎方面有用。
