Feigl Gábor, Varga Viktória, Molnár Árpád, Dimitrakopoulos Panayiotis G, Kolbert Zsuzsanna
Department of Plant Biology, University of Szeged, Közép fasor 52, H6726 Szeged, Hungary.
Department of Environment, University of the Aegean, 81100 Mytilene, Lesbos, Greece.
Antioxidants (Basel). 2020 Sep 7;9(9):837. doi: 10.3390/antiox9090837.
is an endemic species to the serpentine soils of Lesbos Island (Greece). As a nickel (Ni) hyperaccumulator, it possesses an exceptional Ni tolerance; and it can accumulate up to 0.2-2.4% Ni of its leaves' dry weight. In our study, seeds from two geographically separated study sites (Ampeliko and Loutra) were germinated and grown on control and Ni-containing (3000 mg/kg) soil in a rhizotron system. Ni excess induced significant Ni uptake and translocation in both ecotypes and affected their root architecture differently: plants from the Ampeliko site proved to be more tolerant; since their root growth was less inhibited compared to plants originated from the Loutra site. In the roots of the Ampeliko ecotype nitric oxide (NO) was being accumulated, while the degree of protein tyrosine nitration decreased; suggesting that NO in this case acts as a signaling molecule. Moreover, the detected decrease in protein tyrosine nitration may serve as an indicator of this ecotype's better relative tolerance compared to the more sensitive plants originated from Loutra. Results suggest that Ni hypertolerance and the ability of hyperaccumulation might be connected to the plants' capability of maintaining their nitrosative balance; yet, relatively little is known about the relationship between excess Ni, tolerance mechanisms and the balance of reactive nitrogen species in plants so far.
是希腊莱斯博斯岛蛇纹石土壤中的特有物种。作为一种镍（Ni）超积累植物，它具有非凡的镍耐受性；其叶片干重中镍的积累量可达0.2 - 2.4%。在我们的研究中，来自两个地理上分离的研究地点（安佩利科和卢特拉）的种子在根箱系统中的对照土壤和含镍（3000 mg/kg）土壤上发芽并生长。镍过量导致两种生态型植物都显著吸收和转运镍，并对它们的根系结构产生不同影响：来自安佩利科地点的植物耐受性更强；因为与来自卢特拉地点的植物相比，它们的根系生长受到的抑制较小。在安佩利科生态型植物的根中积累了一氧化氮（NO），而蛋白质酪氨酸硝化程度降低；这表明在这种情况下NO作为一种信号分子发挥作用。此外，检测到的蛋白质酪氨酸硝化程度降低可能表明与来自卢特拉的更敏感植物相比，这种生态型植物具有更好的相对耐受性。结果表明，镍超耐受性和超积累能力可能与植物维持其亚硝化平衡的能力有关；然而，到目前为止，关于过量镍、耐受机制与植物活性氮物种平衡之间的关系，人们了解得还相对较少。
