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化学计量学方法测定七种鱼籽的脂肪酸谱和指纹图谱

Fatty-acid Profiles and Fingerprints of Seven Types of Fish Roes as Determined by Chemometric Methods.

作者信息

Ma Shuang, Li Lai Hao, Hao Shu Xian, Yang Xian Qing, Huang Hui, Cen Jian Wei, Wang Yue Qi

机构信息

Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs; Guangdong Provincial Key Laboratory of Fishery Ecology Environment; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences.

College of Food Science and Technology, Shanghai Ocean University.

出版信息

J Oleo Sci. 2020 Oct 7;69(10):1199-1208. doi: 10.5650/jos.ess20061. Epub 2020 Sep 10.

DOI:10.5650/jos.ess20061
PMID:32908092
Abstract

The fatty acids in seven species of fish roes were determined by GC-MS in combination with principal component and cluster analyses in order to derive their fatty-acid profiles and fingerprints. Twenty-three common chromatography peaks were identified in the fatty-acid fingerprints of the seven fish roes. A total of 19 typical fatty acids were identified in the fish roes studied. The fatty acid contents of the roes were significantly different, with saturated-fatty-acid contents in the seven roes ranging from 26.69% to 41.81%, and the unsaturated-fatty-acid contents ranging from 57.65% to 72.21%, the total EPA and DHA content (37.20%) is high in E. cypselurus roe, especially. The seven roe species were clearly distinguished according to fatty-acid composition and content by principal component analysis (PCA) and divided into two groups by cluster analysis (CA). PCA of the fatty acid data yielded three significant PCs , which together account for 94% of the total variance; with PC1 contributing 54% of the total.

摘要

采用气相色谱-质谱联用(GC-MS)结合主成分分析和聚类分析的方法,测定了7种鱼籽中的脂肪酸,以得出其脂肪酸谱和指纹图谱。在7种鱼籽的脂肪酸指纹图谱中鉴定出23个常见的色谱峰。在所研究的鱼籽中共鉴定出19种典型脂肪酸。鱼籽的脂肪酸含量存在显著差异,7种鱼籽中饱和脂肪酸含量在26.69%至41.81%之间,不饱和脂肪酸含量在57.65%至72.21%之间,特别是圆腹鲱鱼籽中EPA和DHA的总含量较高(37.20%)。通过主成分分析(PCA)根据脂肪酸组成和含量可清晰区分这7种鱼籽,并通过聚类分析(CA)将其分为两组。脂肪酸数据的主成分分析产生了三个显著的主成分,它们共同占总方差的94%;其中PC1占总方差的54%。

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