College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
Division of Food Technology, National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.
Food Chem. 2018 Apr 15;245:806-811. doi: 10.1016/j.foodchem.2017.11.108. Epub 2017 Nov 29.
The research aims to study phospholipids (PL) classes and molecular species of large yellow croaker (Pseudosciaena crocea) roe. Both gas chromatographymass spectroscopy (GC-MS) and high-performance liquid chromatography with evaporative light-scattering detection (HPLC-ELSD) were utilized to analyze and identify the PLs fatty acids compositions and classes in the P. crocea roe, respectively. Docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5) account for 35.0% and 6.9% of the PLs. Phosphatidylcholines (PC), lysophosphatidylcholines (LPC), phosphatidylethanolamines (PE) and phosphatidylinositols (PI) account for 76.36 ± 0.62%, 12.30 ± 0.55%, 9.12 ± 0.02% and 1.09 ± 0.01% of the total PLs, respectively. In addition, the PLs molecular species were characterized by ultra-high performance liquid chromatography-electrospray ionization-quadruple-time of flight-mass spectrometry (UPLC-Q-TOF-MS). A total of 92 PLs molecular species was identified, including 49 PCs, 13 PEs, 10 phosphatidic acids (PAs), 13 phosphatidylserines (PSs), 3 phosphatidylglycerols (PGs), 2 sphingomyelins (SMs), and 2 PIs of the P. crocea roe.
本研究旨在研究大黄鱼（Pseudosciaena crocea）鱼卵中的磷脂（PL）种类和分子种类。分别采用气相色谱-质谱联用（GC-MS）和高效液相色谱-蒸发光散射检测（HPLC-ELSD）分析鉴定鱼卵中 PL 的脂肪酸组成和种类。二十二碳六烯酸（DHA，C22:6）和二十碳五烯酸（EPA，C20:5）占 PL 的 35.0%和 6.9%。磷脂酰胆碱（PC）、溶血磷脂酰胆碱（LPC）、磷脂酰乙醇胺（PE）和磷脂酰肌醇（PI）分别占总 PL 的 76.36±0.62%、12.30±0.55%、9.12±0.02%和 1.09±0.01%。此外，还通过超高效液相色谱-电喷雾电离-四极杆飞行时间质谱（UPLC-Q-TOF-MS）对 PL 的分子种类进行了表征。共鉴定出 92 种 PL 分子种类，包括 49 种 PC、13 种 PE、10 种磷脂酸（PA）、13 种磷脂酰丝氨酸（PS）、3 种磷脂甘油（PG）、2 种鞘磷脂（SM）和 2 种 PI。
