Çetinkaya Serap, Yenidünya Ali Fazıl, Başoğlu Faika, Polat Zübeyda Akın, Savaş Sümeyra
Department of Molecular Biology and Genetics, Science Faculty, Sivas Cumhuriyet University.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Europen University of Lefke.
J Oleo Sci. 2020 Oct 7;69(10):1281-1285. doi: 10.5650/jos.ess20142. Epub 2020 Sep 10.
In this study two different strategy were followed to obtain a D-fructose-oleic acid ester. One of the strategies has been well established enzymatic synthesis of an ester bond. The other strategy excluded the biocatalyst and only used a mixture of two organic solvents as the reaction media, 2-methyl-2-butanol / dimethyl sulfoxide or tert-butanol / dimethyl sulfoxide for the production of D-fructose-oleic acid ester. Ester products obtained were characterised by using FT-IR, NMR, by MS. Product yield was also assessed by HPLC. Results of structural analyses and yield measurement indicated that two approaches produced almost identical ester products.
在本研究中，采用了两种不同的策略来获得D-果糖油酸酯。其中一种策略是已成熟的酯键酶促合成法。另一种策略不使用生物催化剂，仅使用两种有机溶剂的混合物作为反应介质，即2-甲基-2-丁醇/二甲基亚砜或叔丁醇/二甲基亚砜来制备D-果糖油酸酯。通过傅里叶变换红外光谱（FT-IR）、核磁共振（NMR）和质谱（MS）对所得酯产物进行表征。还通过高效液相色谱（HPLC）评估产物产率。结构分析和产率测量结果表明，两种方法产生的酯产物几乎相同。
