Honda Kazuhisa, Takagi Shoko, Kurachi Kiyotaka, Sugimoto Haruka, Saneyasu Takaoki, Kamisoyama Hiroshi
Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
J Poult Sci. 2017 Oct 25;54(4):292-295. doi: 10.2141/jpsa.0170004.
The excessive accumulation of body fat has become a serious problem in the broiler industry. However, the molecular mechanisms underlying the regulation of lipid metabolism-related genes in broiler chickens are not fully understood. In the present study, we investigated the role of glucagon on the expression of lipid metabolism-related genes in chicken white adipose tissue (WAT). Four hours of fasting significantly increased plasma levels of free fatty acid in broiler chickens. The mRNA levels of adipose triglyceride lipase (ATGL) and pyruvate dehydrogenase kinase 4 (PDK4) in abdominal WAT significantly increased by fasting, whereas the mRNA levels of diacylglycerol O-acyl-transferase homolog 2 (DGAT2) and peroxisome proliferator-activated receptor- (PPAR) significantly decreased. The results suggest that fasting stimulates lipolysis and suppresses adipogenesis and re-esterification of TG in chicken WAT. Glucagon significantly increased the mRNA levels of PDK4 in chicken primary adipocytes, whereas there were no significant changes in the mRNA levels of ATGL, DGAT2, and PPAR. Our findings suggest that glucagon upregulates PDK4 expression and may stimulate lipolysis without affecting the expression of ATGL in chicken WAT.
体脂过度积累已成为肉鸡养殖业中的一个严重问题。然而，肉鸡中脂质代谢相关基因调控的分子机制尚未完全明确。在本研究中，我们探究了胰高血糖素对鸡白色脂肪组织（WAT）中脂质代谢相关基因表达的作用。禁食4小时显著提高了肉鸡血浆中游离脂肪酸的水平。禁食使腹部WAT中脂肪甘油三酯脂肪酶（ATGL）和丙酮酸脱氢酶激酶4（PDK4）的mRNA水平显著升高，而二酰甘油O-酰基转移酶同源物2（DGAT2）和过氧化物酶体增殖物激活受体-（PPAR）的mRNA水平显著降低。结果表明，禁食刺激鸡WAT中的脂肪分解，抑制脂肪生成和甘油三酯（TG）的再酯化。胰高血糖素显著提高了鸡原代脂肪细胞中PDK4的mRNA水平，而ATGL、DGAT2和PPAR的mRNA水平没有显著变化。我们的研究结果表明，胰高血糖素上调PDK4表达，可能刺激鸡WAT中的脂肪分解，而不影响ATGL的表达。
