Sox10 在 Wistar 大鼠免疫组化染色中是神经嵴干细胞的特异性标志物。
Sox10 Is a Specific Biomarker for Neural Crest Stem Cells in Immunohistochemical Staining in Wistar Rats.
机构信息
Department of Pediatric Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, China.
Department of Pediatric Surgery, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China.
出版信息
Dis Markers. 2020 Aug 28;2020:8893703. doi: 10.1155/2020/8893703. eCollection 2020.
OBJECTIVE
Neural crest stem cells (NCSCs) are prototypically migratory cells immigrating from the dorsal neural tube to specific embryonic sites where they generate a variety of cell types. A lot of biomarkers for NCSCs have been identified. However, which biomarkers are the most specific is still unclear.
METHODS
The rat embryos harvested in embryonic day 9 (E9), E9.5, E10, E10.5, E11, E12, E13, and E14 were paraffin-embedded and sectioned in transverse. NCSCs were spatiotemporally demonstrated by immunohistochemical staining with RET, p75NTR, Pax7, and Sox10. NCSCs were isolated, cultured, and stained with RET, p75NTR, Pax7, and Sox10.
RESULTS
In the paraffin sections of rat embryos, the immunohistochemical staining of RET, p75NTR, and Sox10 can all be used in demonstrating NCSCs. Sox10 was positive mainly in NCSCs while RET and p75NTR were positive not only in NCSCs but also in other tissue cells. In primary culture cells, Sox10 was mainly in the nucleus of NCSCs, RET was mainly in the membrane, and p75NTR was positive in cytoplasm and membrane.
CONCLUSIONS
Sox10 is the specific marker for immunohistochemical staining of NCSCs in paraffin sections. In cultured cells, Sox10, p75NTR, and RET presented a similar staining effect.
目的
神经嵴干细胞（NCSCs）是典型的迁移细胞，从背侧神经管迁移到特定的胚胎部位，在那里它们产生各种细胞类型。已经鉴定出许多 NCSCs 的生物标志物。然而，哪些生物标志物最具特异性仍不清楚。
方法
在胚胎第 9 天（E9）、E9.5、E10、E10.5、E11、E12、E13 和 E14 收获的大鼠胚胎进行石蜡包埋并横向切片。通过用 RET、p75NTR、Pax7 和 Sox10 进行免疫组织化学染色来时空显示 NCSCs。分离、培养 NCSCs，并对其进行 RET、p75NTR、Pax7 和 Sox10 的染色。
结果
在大鼠胚胎的石蜡切片中，RET、p75NTR 和 Sox10 的免疫组织化学染色均可用于显示 NCSCs。Sox10 主要在 NCSCs 中呈阳性，而 RET 和 p75NTR 不仅在 NCSCs 中呈阳性，而且在其他组织细胞中也呈阳性。在原代培养细胞中，Sox10 主要位于 NCSCs 的核内，RET 主要位于膜上，p75NTR 阳性位于细胞质和膜上。
结论
Sox10 是石蜡切片中 NCSCs 免疫组织化学染色的特异性标志物。在培养细胞中，Sox10、p75NTR 和 RET 呈现出相似的染色效果。
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