Department of Medical Biology, Faculty of Medicine, Atatürk University, Erzurum, Turkey.
Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey.
Biol Trace Elem Res. 2021 Jul;199(7):2602-2611. doi: 10.1007/s12011-020-02374-7. Epub 2020 Sep 9.
Nickel boride is generally used in the steel industry as a melting accelerator due to its feature of creating a protective and stable attribute at high temperatures. It is also used to improve the hardenability of the steel with boron addition in the production. Thus, safety studies and biocompatibility analysis of nickel boride should be performed comprehensively to understand the limitations of use in various areas. In the present study, nickel boride nanoparticles (NiB NPs) were synthesized by a single-step method and molecule characterizations were performed via the use of X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) analyses. Cytotoxicity properties of NiB NPs were identified on human pulmonary alveolar epithelial cells (HPAEpiC) by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), neutral red (NR), and lactate dehydrogenase (LDH) assays. Illumina human ht-12 v4.0 whole-genome microarray analysis was conducted to investigate NiB NPs effects on gene expression regulations of HPAEpiC cells. The database for annotation, visualization, and integrated discovery (DAVID) analysis was performed to reveal the relationship between NiB NP application and cellular pathway alterations. According to cytotoxicity analysis, the IC value for NiB NP application was found as 81.99 mg/L concentration. Microarray analysis of NiB NP application was shown for the first time that 693 gene expression changes (FC ≥ 2) occurred significantly over 40.000 gene probes and NiB NPs were observed to affect microtubule regulation, centrosome organization, and phosphoprotein synthesis.
硼化镍通常在钢铁行业中用作熔炼促进剂，因为它在高温下具有产生保护和稳定特性的特点。它还用于通过在生产中添加硼来提高钢的淬透性。因此，应该全面进行镍硼化物的安全性研究和生物相容性分析，以了解其在各个领域的使用限制。在本研究中，通过一步法合成了镍硼纳米粒子（NiB NPs），并通过 X 射线衍射分析（XRD）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）和能谱分析（EDX）进行了分子表征。通过使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）、中性红（NR）和乳酸脱氢酶（LDH）测定法，在人肺泡上皮细胞（HPAEpiC）上鉴定了 NiB NPs 的细胞毒性特性。进行了 Illumina 人类 ht-12 v4.0 全基因组微阵列分析，以研究 NiB NPs 对 HPAEpiC 细胞基因表达调控的影响。对数据库进行了注释、可视化和综合发现（DAVID）分析，以揭示 NiB NP 应用与细胞途径改变之间的关系。根据细胞毒性分析，发现 NiB NP 应用的 IC 值为 81.99 mg/L 浓度。NiB NP 应用的微阵列分析首次表明，超过 40,000 个基因探针中发生了 693 个基因表达变化（FC≥2），并且观察到 NiB NPs 影响微管调节、中心体组织和磷酸蛋白合成。
