Liu Yan, Jin Haijia, Zou Wenting, Guo Rong
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, P. R. China.
J Mater Chem B. 2020 Sep 10. doi: 10.1039/d0tb01721c.
Developing a multifunctional nanozyme based biosensor with a convenient approach and high reliability is of vital interest for multiplex detection. In this study, wool-ball-like copper sulfide (WBLCS) was obtained facilely using an amphiphilic protein. The acidic amino acid residues and the amphiphilic properties of protein molecules play cooperative roles in the fabrication of hierarchical nanostructures. Unlike copper sulfide with irregular morphologies, the single component WBLCS acts as a multifunctional nanozyme possessing both superior cysteine oxidase- and peroxidase-mimicking activity. Fascinatingly, the addition of silver ions can significantly enhance the performance of the cascade system at a very low fluorescence substrate concentration. Based on this, dual fluorescence "turn on" sensors of cysteine and silver ions with extremely high sensitivity and selectivity are developed. This is the first report to explore multiple fluorescence "turn on" sensing systems based on one single nanozyme. Hence, the present finding has significant implication towards the design of superstructured nanozymes combining different multi-functionalities at the nanoscale for sensing multiplex target molecules sensitively and selectively in practice.
开发一种具有便捷方法和高可靠性的基于多功能纳米酶的生物传感器对于多重检测至关重要。在本研究中，使用两亲性蛋白质轻松获得了羊毛球状硫化铜（WBLCS）。蛋白质分子的酸性氨基酸残基和两亲性在分级纳米结构的制备中发挥协同作用。与具有不规则形态的硫化铜不同，单一组分的WBLCS作为一种多功能纳米酶，具有优异的模拟半胱氨酸氧化酶和过氧化物酶的活性。令人着迷的是，在非常低的荧光底物浓度下，添加银离子可以显著提高级联系统的性能。基于此，开发了具有极高灵敏度和选择性的半胱氨酸和银离子双荧光“开启”传感器。这是首次探索基于单一纳米酶的多重荧光“开启”传感系统的报告。因此，本发现对于在纳米尺度上结合不同多功能性以在实际中灵敏且选择性地检测多重目标分子的超结构纳米酶的设计具有重要意义。
