Liu Yan, Jin Haijia, Zou Wenting, Guo Rong
School of Chemistry and Chemical Engineering, Yangzhou University Yangzhou 225002 Jiangsu P. R. China
RSC Adv. 2020 Aug 4;10(48):28819-28826. doi: 10.1039/d0ra05496h. eCollection 2020 Aug 3.
Strenuous efforts have been made to develop nanozymes for achieving the performance of natural enzymes to broaden their application in practice, but the fabrication of high-performance and biocompatible nanozymes facile and versatile approaches has always been a great challenge. Here, sponge-like casein-CuS hybrid has been facilely synthesized in the presence of amphiphilic protein-casein through a simple one-step approach. Casein-CuS hybrid exhibits substrates-dependent peroxidase-like activity. Casein-CuS hybrid exhibits well peroxidase-like activity with 3,3',5,5'-tetramethylbenzidine (TMB) and 1,2-diaminobenzene (OPD) as substrates, and the affinity of OPD towards the hybrid nanozyme is much higher than that of TMB. More importantly, due to the high affinity of OPD and the well biocompatibility of the hybrid nanozyme, a superior enzyme cascade for glucose based on the well cooperative effect of casein-CuS hybrid and glucose oxidase is developed. The proposed glucose sensor exhibits a wide linear range of 0.083 to 75 μM and a detection limit of 5 nM. This suggests the promising utilization of protein-metal hybrid nanozymes as robust and potent peroxidase mimics in the medical, food and environmental detection fields.
人们已付出巨大努力来开发纳米酶，以实现天然酶的性能，从而拓宽其实际应用范围，但制备高性能且生物相容的纳米酶的简便通用方法一直是巨大挑战。在此，通过一种简单的一步法，在两亲性蛋白质 - 酪蛋白存在的情况下轻松合成了海绵状酪蛋白 - 硫化铜杂化物。酪蛋白 - 硫化铜杂化物表现出底物依赖性类过氧化物酶活性。以3,3',5,5'-四甲基联苯胺（TMB）和邻苯二胺（OPD）为底物时，酪蛋白 - 硫化铜杂化物表现出良好的类过氧化物酶活性，且OPD对杂化纳米酶的亲和力远高于TMB。更重要的是，由于OPD的高亲和力以及杂化纳米酶良好的生物相容性，基于酪蛋白 - 硫化铜杂化物与葡萄糖氧化酶的良好协同效应，开发了一种用于葡萄糖的优异酶级联反应。所提出的葡萄糖传感器具有0.083至75 μM的宽线性范围和5 nM的检测限。这表明蛋白质 - 金属杂化纳米酶作为强大且有效的过氧化物酶模拟物在医学、食品和环境检测领域具有广阔的应用前景。
