Besemer J, Molzberger C
Mol Gen Genet. 1977 May 20;153(1):51-60. doi: 10.1007/BF01035996.
Insertion elements IS1 and IS2 integrated within the gal operator-promoter region, an IS1 element in gene galT and insertions IS1 and IS2 integrated in the xycIIOP region of phage lambda were transcribed in vitro with E. coli RNA-polymerase. The insertion elements are transcribed exclusively by polymerase molecules started at the gal promoter and the lambdaPR promoter respectively. No promoter exists on IS1 or IS2 which can be recognized by RNA-polymerase in the pure in vitro transcription system used. Both insertions apparently are transcribed with a lower elongation rate than gal operon DNA or lambdaDNA. RNAs transcribed from the termini of IS1 and IS2 respectively were analysed by hybridization experiments. They are different in sequence.
插入元件IS1和IS2整合在gal操纵子 - 启动子区域内，基因galT中的一个IS1元件以及整合在噬菌体λ的xycIIOPOPOP区域中的IS1和IS2插入片段，在体外由大肠杆菌RNA聚合酶进行转录。这些插入元件分别由起始于gal启动子和λPR启动子的聚合酶分子专门转录。在所使用的纯体外转录系统中，IS1或IS2上不存在可被RNA聚合酶识别的启动子。这两个插入片段的转录延伸速率显然都低于gal操纵子DNA或λDNA。通过杂交实验分析了分别从IS1和IS2末端转录的RNA。它们的序列不同。
