Department of Chemical Engineering & Technology, Indian Institute of Technology (Banaras Hindu University) Varanasi, Varanasi, Uttar Pradesh 221005, India.
Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology (Banaras Hindu University) Varanasi, Varanasi, Uttar Pradesh 221005, India.
Int J Pharm. 2020 Nov 15;589:119858. doi: 10.1016/j.ijpharm.2020.119858. Epub 2020 Sep 7.
Open deep cuts and wounds often take a long time to heal and may cause infection and scar formation. A simple biomimetic electrospun nano-fibrous antimicrobial dressing material loaded with dual anti-oxidants has been developed to address this problem. A composite nano-fibrous material (PVP-Ce-Cur NF) comprising polyvinyl pyrrolidone (PVP), cerium nitrate hexahydrate (Ce(NO)·6HO) and curcumin was developed using the electrospinning technique. The surface characteristics of nanofibers were examined using standard analytical techniques. The antimicrobial activity was tested against Escherichia coli (gram -ve) and Staphylococcus aureus (gram +ve) bacterial species. The MTT assay using 3T6-Swiss albino fibroblast indicated that the nano-fibrous dressing material was hemocompatible and devoid of cytotoxicity. Direct application of the PVP-Ce-Cur NF dressing material on full-thickness circular excision wound in model wistar rats displayed complete healing and re-epithelialisation without any scar within 20 days. A well-regulated level of hydroxyproline, superoxide dismutase (SOD) and catalase in granulation tissues indicated PVP-Ce-Cur NF as a potential wound dressing material with the anti-scar property.
开放性深切口和创伤通常需要很长时间才能愈合，并且可能导致感染和瘢痕形成。已经开发出一种简单的仿生电纺纳米纤维抗菌敷料材料，该材料负载有双重抗氧化剂，以解决这个问题。采用静电纺丝技术制备了由聚乙烯吡咯烷酮（PVP）、六水硝酸铈（Ce（NO）·6HO）和姜黄素组成的复合纳米纤维材料（PVP-Ce-Cur NF）。使用标准分析技术检查了纳米纤维的表面特性。对大肠杆菌（革兰氏阴性）和金黄色葡萄球菌（革兰氏阳性）细菌进行了抗菌活性测试。使用 3T6-瑞士白化纤维母细胞的 MTT 测定表明，纳米纤维敷料材料具有血液相容性，并且没有细胞毒性。在 Wistar 大鼠模型的全层圆形切除伤口上直接应用 PVP-Ce-Cur NF 敷料材料，在 20 天内显示出完全愈合和再上皮化，没有任何瘢痕。肉芽组织中羟脯氨酸、超氧化物歧化酶（SOD）和过氧化氢酶的水平调节良好，表明 PVP-Ce-Cur NF 是一种具有抗瘢痕特性的潜在伤口敷料材料。
