Safta Diana Antonia, Vlase Ana-Maria, Pop Anca, Cherfan Julien, Carpa Rahela, Iurian Sonia, Bogdan Cătălina, Vlase Laurian, Moldovan Mirela-Liliana
Department of Dermopharmacy and Cosmetics, Faculty of Pharmacy, "Iuliu Haţieganu" University of Medicine and Pharmacy, 12 I. Creangă St., 400010 Cluj-Napoca, Romania.
Department of Pharmaceutical Botany, Faculty of Pharmacy, "Iuliu Haţieganu" University of Medicine and Pharmacy, 8 Victor Babes Street, 400012 Cluj-Napoca, Romania.
Antioxidants (Basel). 2025 Apr 27;14(5):521. doi: 10.3390/antiox14050521.
This study aimed to optimize the extraction of phytocompounds intended for wound care applications from three plant species, L. flowers and L. and L. aerial parts, by using a Quality by Design approach. The effects of different extraction methods (ultra-turrax and ultrasonic-assisted extraction), ethanol concentrations (30%, 50%, 70%), and extraction times (3, 5, 10 min) were studied, and during the optimization step, the polyphenol and flavonoid contents were maximized. The phytochemical profiles of the optimized HEs (herbal extracts) were assessed using LC-MS/MS methods. The antioxidant capacity of the optimized HEs was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity) TEAC (Trolox equivalent antioxidant capacity), and FRAP (ferric reducing antioxidant power) assays, while the antibacterial activity was evaluated against , , and MSSA-methicillin-sensitive and MRSA-methicillin-resistant ). Cell viability and antioxidant and wound healing potential were assessed on keratinocytes and fibroblasts. The anti-inflammatory effect was assessed on fibroblasts by measuring levels of interleukins IL-6 and IL-8 and the production of nitric oxide from RAW 264.7 cells. The major compounds of the optimized HEs were rutin and chlorogenic acid. The optimized HE showed the strongest antibacterial activity, while the optimized HE demonstrated high cell viability. and optimized HEs showed increased antioxidant capacities. All extracts displayed anti-inflammatory effects, and the optimized HE exhibited the best in vitro wound-healing effect.
本研究旨在采用质量源于设计的方法，优化从三种植物（L.花、L.地上部分和L.地上部分）中提取用于伤口护理的植物化合物的工艺。研究了不同提取方法（超高速匀浆法和超声辅助提取法）、乙醇浓度（30%、50%、70%）和提取时间（3、5、10分钟）的影响，并在优化步骤中使多酚和黄酮含量最大化。使用LC-MS/MS方法评估优化后的草药提取物（HEs）的植物化学特征。使用DPPH（2,2-二苯基-1-苦基肼自由基清除能力）、TEAC（Trolox当量抗氧化能力）和FRAP（铁还原抗氧化能力）测定法测定优化后的HEs的抗氧化能力，同时评估其对大肠杆菌、金黄色葡萄球菌-甲氧西林敏感菌（MSSA）和金黄色葡萄球菌-甲氧西林耐药菌（MRSA）的抗菌活性。在角质形成细胞和成纤维细胞上评估细胞活力、抗氧化和伤口愈合潜力。通过测量白细胞介素IL-6和IL-8的水平以及RAW 264.7细胞中一氧化氮的产生，评估成纤维细胞的抗炎作用。优化后的HEs的主要化合物为芦丁和绿原酸。优化后的HE显示出最强的抗菌活性，而优化后的HE表现出高细胞活力。优化后的HEs显示出增强的抗氧化能力。所有提取物均显示出抗炎作用，优化后的HE表现出最佳的体外伤口愈合效果。
