Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Tehran, Iran.
Industrial and Environmental Biotechnology Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Protein Expr Purif. 2021 Jan;177:105748. doi: 10.1016/j.pep.2020.105748. Epub 2020 Sep 7.
The antioxidant activity and cell viability of feather hydrolysates obtained with the Bacillus licheniformis were evaluated using an in-vitro model. The results indicate that feathers-derived peptides under 3 kDa have antioxidant activity with IC values of 5.03 ± 0.215 mg/mL by using DPPH antioxidant assay. Although the antioxidant activity of the peptides under 3 kDa preserved after applying diverse heating (from 20 to 100 °C), they lost their activity under strongly acidic or alkaline conditions. Antioxidant activity of the mixed feather bioactive peptides (MFBPs) obtained with partial purification of peptides under 3 kDa was with IC amount of 0.169 mg/mL ± 0.004 using DPPH radical scavenging assay. Also, MFBPs within an amount range of from 0.0048 to 5.0 mg/mL, illustrated no cytotoxicity to gingival fibroblast blood cell lines. In light of our results, the obtained value-added peptides could be useful in different food products as a future functional ingredient with antioxidant potency.
采用枯草芽孢杆菌对羽毛进行水解，得到的水解产物的抗氧化活性和细胞活力采用体外模型进行了评估。结果表明，采用 DPPH 抗氧化测定法，3 kDa 以下的羽毛衍生肽的 IC 值为 5.03±0.215mg/mL，具有抗氧化活性。尽管在应用不同的加热（20-100°C）后，3 kDa 以下的肽的抗氧化活性得以保留，但在强酸或强碱条件下它们会失去活性。采用部分纯化 3 kDa 以下肽得到的混合羽毛生物活性肽（MFBPs）的抗氧化活性，用 DPPH 自由基清除测定法，IC 值为 0.169mg/mL±0.004。此外，在 0.0048 至 5.0mg/mL 范围内的 MFBPs 对牙龈成纤维细胞系没有细胞毒性。根据我们的结果，所得的增值肽可用作具有抗氧化能力的未来功能性成分，应用于不同的食品产品中。
