Industrial and Environmental Biotechnology Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
World J Microbiol Biotechnol. 2024 Mar 5;40(4):123. doi: 10.1007/s11274-024-03921-3.
Bacteria have the potential to adhere to abiotic surfaces, which has an undesirable effect in the food industry because they can survive for sustained periods through biofilm formation. In this study, an antibacterial peptide (ABP), with a molecular mass of 3861 Da, was purified from hydrolyzed chicken feathers using a locally isolated keratinolytic bacterium, namely Rhodococcus erythropolis, and its antibacterial and antibiofilm potential were investigated against planktonic and biofilm cells of Methicillin-Resistant Staphylococcus Aureus (MRSA). The results demonstrated that purified ABP showed the growth inhibition of MRSA cells with the minimum inhibitory concentration (MIC) of 45 µg/ml and disrupted MRSA biofilm formation at a concentration of 200 ug/ml, which results were confirmed by scanning electron micrograph (SEM). Moreover, the secondary structures of the peptide were assessed as part of the FTIR analysis to evaluate its mode of action. ExPASy tools were used to predict the ABP sequence, EPCVQUQDSRVVIQPSPVVVVTLPGPILSSFPQNTA, from a chicken feather keratin sequence database following in silico digestion by trypsin. Also, ABP had 54.29% hydrophobic amino acids, potentially contributing to its antimicrobial activity. The findings of toxicity prediction of the peptide by the ToxinPred tool revealed that ABP had non-toxic effects. Thus, these results support the potential of this peptide to be used as an antimicrobial agent for the treatment or prevention of MRSA biofilm formation in feed, food, or pharmaceutical applications.
细菌有附着在非生物表面的潜力，这在食品工业中是一个不利的影响，因为它们可以通过生物膜形成而持续生存。在这项研究中，一种抗菌肽（ABP），分子量为 3861 Da，从水解的鸡毛中被分离出来，使用的是一种当地分离的角蛋白分解菌，即红色糖多孢菌，其抗菌和抗生物膜潜力被针对耐甲氧西林金黄色葡萄球菌（MRSA）的浮游细胞和生物膜细胞进行了研究。结果表明，纯化的 ABP 对 MRSA 细胞表现出生长抑制作用，最小抑菌浓度（MIC）为 45 µg/ml，在 200 ug/ml 的浓度下破坏 MRSA 生物膜形成，这些结果通过扫描电子显微镜（SEM）得到证实。此外，肽的二级结构作为 FTIR 分析的一部分进行评估，以评估其作用模式。ExPASy 工具用于从鸡毛角蛋白序列数据库中预测 ABP 序列，EPCVQUQDSRVVIQPSPVVVVTLPGPILSSFPQNTA，在经过胰蛋白酶的计算机模拟消化之后。此外，ABP 有 54.29%的疏水性氨基酸，可能有助于其抗菌活性。肽的毒性预测工具 ToxinPred 的结果表明 ABP 没有毒性作用。因此，这些结果支持该肽作为治疗或预防饲料、食品或制药应用中 MRSA 生物膜形成的抗菌剂的潜力。
