College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163316, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, 130118, China.
Service Center of Luohe Inspection Area, Designated Port of Imported Meat Products in Henan Province, Luohe, 46200, China.
Fish Shellfish Immunol. 2020 Nov;106:993-1003. doi: 10.1016/j.fsi.2020.09.005. Epub 2020 Sep 7.
Chromium (Cr) is the most common heavy metal and that becomes toxic when present at higher concentrations in aquatic environments. Allium mongolicum Regel flavonoids (AMRF) has been documented to possess detoxification, antioxidant and anti-inflammatory properties. The aim of this study was to explore the potential of dietary AMRF and Cr exposure on bioaccumulation, oxidative stress, and immune response in Ctenopharyngodon idella. After acclimation, 360 fish were randomly distributed into six groups. The fish were fed with diets supplemented with Cr and/or AMRF for 4 weeks (28 days), the Cr concentrations were 0, 120, and 240 mg/kg and the concentrations of AMRF were 0 or 40 mg/kg, respectively. The results shown that Cr accumulation in the kidney, liver, spleen, intestine and gill were significantly increased following Cr exposure, dietary AMRF supplementation attenuated the increased in Cr accumulation. Dietary AMRF supplementation significantly reduced the levels of malondialdehyde (MDA) and protein carbonyl (PC) in liver, spleen and gill compared with the same Cr dose groups. When fish were supplemented with AMRF significantly increased lysozyme activity (LZM), complement 3 (C3) in kidney and intestine compared with the same Cr dose groups. Serum glutamic oxalate transaminase (GOT) and glutamic pyruvate transaminase (GPT) were significantly increased following exposure to Cr. Dietary AMRF supplementation significantly decreased GOT and GPT activity in the serum. In addition, AMRF supplementation can decrease the expression of inflammatory (NF-κB p65, TNF-α and IL-1β) and increased the expression of tight junction proteins (occludin and ZO-1) following Cr exposure in C. idella. These results indicate that AMRF has the potential to alleviate the effects of Cr toxicity in C. idella.
铬（Cr）是最常见的重金属，当在水生环境中浓度较高时，会变得有毒。已经有文献记载，蒙古黄芪黄酮（AMRF）具有解毒、抗氧化和抗炎作用。本研究旨在探讨膳食 AMRF 和 Cr 暴露对鲤鱼（Ctenopharyngodon idella）生物蓄积、氧化应激和免疫反应的潜在影响。经过适应期后，将 360 条鱼随机分配到 6 个组中。这些鱼分别用添加 Cr 和/或 AMRF 的饮食喂养 4 周（28 天），Cr 的浓度分别为 0、120 和 240mg/kg，AMRF 的浓度分别为 0 或 40mg/kg。结果表明，Cr 暴露后，肾脏、肝脏、脾脏、肠和鳃中的 Cr 蓄积显著增加，膳食 AMRF 补充可减轻 Cr 蓄积的增加。与相同 Cr 剂量组相比，膳食 AMRF 补充可显著降低肝脏、脾脏和鳃中的丙二醛（MDA）和蛋白质羰基（PC）水平。与相同 Cr 剂量组相比，补充 AMRF 可显著增加肾脏和肠道中的溶菌酶活性（LZM）和补体 3（C3）。Cr 暴露后，血清谷氨酸草酰乙酸转氨酶（GOT）和谷氨酸丙酮酸转氨酶（GPT）显著升高。膳食 AMRF 补充可显著降低血清中 GOT 和 GPT 的活性。此外，AMRF 补充可降低 Cr 暴露后鲤鱼（C. idella）中炎症（NF-κB p65、TNF-α 和 IL-1β）相关基因的表达，并增加紧密连接蛋白（occludin 和 ZO-1）的表达。这些结果表明，AMRF 具有减轻 Cr 毒性对鲤鱼（C. idella）影响的潜力。
