Tkadlecek L, Viklická S, Hofer M, Karpfel Z
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Strahlenther Onkol. 1988 Jun;164(6):357-62.
The experiment was aimed at studying two contradictory actions: damage to hemopoietic organs during application of radiation doses per fraction and regenerative efforts of the organism supported by repeated bone marrow transplantation. The mice received doses of 3 Gy of 60Co-gamma rays total body irradiation at four-day intervals up to a total dose of 18 Gy. After each dose per fraction half of the animals were injected with 10(6) bone marrow cells. At four-day intervals evaluations were made of the blood count, bone marrow and spleen cellularities, and spleen mass. In animals subjected only to irradiation the damage to hemopoietic organs was becoming deeper until the end of observation. In bone marrow recipients the decrease in bone marrow cellularity and the number of leukocytes stopped after day 16, and there was a steep growth of splenic cellularity and mass; the decrease in erythrocyte count stopped on day 20. Obviously a certain recovery of hemopoiesis occurred after the total dose of 12 Gy between days 12 and 16. The lodging of injected hemopoietic stem cells and their proliferation had to be preceded by a regeneration of the hemopoietic microenvironment.
分次给予辐射剂量时对造血器官的损伤以及通过重复骨髓移植得到机体支持的再生作用。小鼠每隔四天接受一次3 Gy的60Co-γ射线全身照射，直至总剂量达到18 Gy。每次分次剂量照射后，将一半动物注射10(6)个骨髓细胞。每隔四天对血细胞计数、骨髓和脾脏细胞密度以及脾脏质量进行评估。仅接受照射的动物，造血器官的损伤在观察期结束前不断加重。接受骨髓移植的动物，骨髓细胞密度和白细胞数量在第16天后停止下降，脾脏细胞密度和质量急剧增加；红细胞计数在第20天停止下降。显然，在第12天至16天之间总剂量达到12 Gy后，造血功能出现了一定程度的恢复。注入的造血干细胞的着床及其增殖必须先有造血微环境的再生。
