Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
Semin Cell Dev Biol. 2021 May;113:57-64. doi: 10.1016/j.semcdb.2020.08.009. Epub 2020 Sep 8.
TopBP1/Rad4/Dpb11 is an essential eukaryotic protein with important roles in DNA replication, DNA repair, DNA damage checkpoint activation, and chromosome segregation. TopBP1 serves as a scaffold to assemble protein complexes in a phosphorylation-dependent manner via its multiple BRCT-repeats. Recently, it has become clear that TopBP1 is repurposed to scaffold different processes dependent on cell cycle regulated changes in phosphorylation of client proteins. Here we review the functions of human TopBP1 in maintaining genome integrity during mitosis.
TopBP1/Rad4/Dpb11 是一种重要的真核蛋白，在 DNA 复制、DNA 修复、DNA 损伤检查点激活和染色体分离中发挥重要作用。TopBP1 作为一个支架，通过其多个 BRCT 重复序列，以磷酸化依赖的方式组装蛋白复合物。最近，人们已经清楚地认识到，TopBP1 被重新用于支架不同的过程，这取决于细胞周期调控的客户蛋白磷酸化的变化。在这里，我们回顾了人 TopBP1 在有丝分裂过程中维持基因组完整性的功能。
