Bioprocess and Metabolic Engineering Laboratory, Department of Food Engineering, Faculty of Food Engineering, University of Campinas, Campinas, SP, Brazil.
Laboratory of Protein Biotechnology, Department of Chemistry, Faculty of Philosophy Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Enzyme Microb Technol. 2020 Oct;140:109647. doi: 10.1016/j.enzmictec.2020.109647. Epub 2020 Aug 18.
Chimeras with improved properties can be designed by synergistically incorporating multiple proteins into one peptide chain. Such design enhances hydrolysis efficiency and lowers enzyme production costs for numerous applications, particularly in the fields of lignocellulosic biomass hydrolysis, plant-based industries, food additives, waste treatment, and biofuels. In this minireview, we summarize a brief, common approach to construct recombinant enzymes, especially in the Pichia pastoris host. We present the design strategies and the desired properties of chimeras and provide examples of recent studies in the area of lignocellulosic biomass hydrolysis. Subsequently, we describe the progress in structure prediction tools that facilitate the design of chimeric proteins toward identifying the best combinations of fusion partners and linkers.
通过将多种蛋白质协同地整合到一条肽链中，可以设计出具有改良性能的嵌合体。这种设计提高了水解效率，降低了酶的生产成本，适用于许多应用，特别是在木质纤维素生物质水解、植物产业、食品添加剂、废物处理和生物燃料等领域。在这篇综述中，我们总结了构建重组酶的一种简要、通用的方法，特别是在毕赤酵母宿主中。我们提出了嵌合体的设计策略和所需的特性，并提供了木质纤维素生物质水解领域的最新研究实例。随后，我们描述了结构预测工具的进展，这些工具有助于设计嵌合蛋白，以确定融合伙伴和连接子的最佳组合。
