Singh Deepika, Yadav Ekta, Kumar Vikas, Verma Amita
Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad-211007, Uttar Pradesh, India.
Curr Drug Deliv. 2021;18(5):634-644. doi: 10.2174/1567201817666200910154301.
Madhuca longifolia has been used for the treatment of renal cancer. Therefore, the current study describes the protective effects of biofabricated silver nanoparticles (MLAg- NPs) using Madhuca longifolia aqueous leaves extract against diethylnitrosamine (DEN) induced Renal Cell Carcinoma (RCC) in rats.
Animals were categorized into five groups and treated with doses of silver nanoparticles for 16 weeks. Antineoplastic effect in renal cancer was dose dependent to control the macroscopical variations when compared to DEN induced group. Significant changes were observed in biochemical parameters and dose graded improvement in the level of antioxidants parameters were accountable for its protective nature.
Silver nanoparticles in dose dependent manner was effective to modify the raised levels of pro-inflammatory cytokines and inflammatory mediators during renal cancer. Alteration in renal histopathology were also detected in the silver nanoparticles treated group, which show its safety concern. Biofabricated silver nanoparticles (MLAgNPs) using Madhuca longifolia can convey significant chemo-protective effect against renal cancer by suppressing the IL-6, TNF-α and IL-1β by nuclear factor-kappa B (NF-κB) pathway.
Our outcomes implicates that biofabricated MLAgNPs exhibited a chemoprotective potential in the prevention and intervention of RCC.
长叶马胡卡已被用于治疗肾癌。因此，本研究描述了利用长叶马胡卡叶水提取物生物合成的银纳米颗粒（MLAg-NPs）对二乙基亚硝胺（DEN）诱导的大鼠肾细胞癌（RCC）的保护作用。
将动物分为五组，用不同剂量的银纳米颗粒处理16周。与DEN诱导组相比，肾癌的抗肿瘤作用呈剂量依赖性，以控制宏观变化。在生化参数方面观察到显著变化，抗氧化剂参数水平的剂量分级改善是其具有保护性质的原因。
银纳米颗粒以剂量依赖的方式有效改变肾癌期间促炎细胞因子和炎症介质升高的水平。在银纳米颗粒处理组中也检测到肾脏组织病理学的改变，这显示了其安全性问题。利用长叶马胡卡生物合成的银纳米颗粒（MLAgNPs）可通过核因子-κB（NF-κB）途径抑制IL-6、TNF-α和IL-1β，对肾癌具有显著的化学保护作用。
我们的结果表明，生物合成的MLAgNPs在预防和干预RCC方面具有化学保护潜力。
