Bio-organic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, Uttar Pradesh, 211007, India.
Natural Product Drug Discovery Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, Uttar Pradesh, 211007, India.
Inflammopharmacology. 2019 Oct;27(5):1037-1054. doi: 10.1007/s10787-018-0525-6. Epub 2018 Sep 15.
Oxidative stress and inflammation play a pivotal role in the expansion and progression of hepatic cancer. Nanoparticle-based drug delivery can quickly enhance the restorative capability of hepatic cancer. Silver nanoparticles synthesized from plant source are of great importance due to their small size, economic, non-hazardous and different biomedical applications. In the current study, we have evaluated the impacts of oxidative stress and proinflammatory markers of biosynthesized silver nanoparticles of Phyllanthus emblica (PE) leaves against diethylnitrosamine-induced hepatocellular carcinoma (HCC) in wistar rats till 16 weeks with its underlying mechanism. The physico-chemical properties of biosynthesized silver nanoparticles were determined by ultra-visible spectroscopy, Fourier transform infrared spectroscopy, field emission scanning electron microscope, energy dispersive X-ray analysis, transmission electron microscopy and X-ray diffraction studies. Biofabricated silver nanoparticles (PEAgNPs) significantly enhanced the process of recovery from hepatic cancer in animal models, which was ascertained by increased body weight, reduced hepatic knobs on the outer surface of liver, downregulated serum biochemical parameters (ALT: 134.66 ± 2.60; AST: 120.33 ± 3.18; ALP: 153.33 ± 4.25; AFP: 167.33 ± 3.38), decreased hepatic lipid peroxidation (20.22 ± 1.74), increased membrane-bound enzymes (Na/KATPase: 4.18 ± 0.20; CaATPase: 6.24 ± 0.12), increased antioxidants parameters (CAT: 64.89 ± 4.13; SOD: 6.01 ± 0.11; GPx: 8.55 ± 0.05), alteration in the level of proinflammatory cytokines (TNF-α: 90.15 ± 5.77; NF-κB: 173.29 ± 7.26; IL-6: 178.11 ± 3.16; IL-1β: 48.26 ± 1.89) and histopathological studies. Our outcomes implicate successfully biofabrication of silver nanoparticles and exhibited a chemoprotective potential in the prevention and intervention of hepatocellular carcinoma.
氧化应激和炎症在肝癌的扩张和进展中起着关键作用。基于纳米粒子的药物输送可以快速增强肝癌的修复能力。植物源合成的银纳米粒子因其尺寸小、经济、无毒和不同的生物医学应用而非常重要。在目前的研究中，我们评估了叶下珠（PE）叶生物合成的银纳米粒子对二乙基亚硝胺诱导的 Wistar 大鼠肝癌（HCC）的氧化应激和促炎标志物的影响，直到 16 周，并探讨了其潜在机制。通过紫外可见光谱、傅里叶变换红外光谱、场发射扫描电子显微镜、能量色散 X 射线分析、透射电子显微镜和 X 射线衍射研究来确定生物合成的银纳米粒子的物理化学性质。生物制备的银纳米粒子（PEAgNPs）显著增强了动物模型中肝癌恢复的过程，这是通过增加体重、减少肝脏外表面的肝结节、下调血清生化参数（ALT：134.66±2.60；AST：120.33±3.18；ALP：153.33±4.25；AFP：167.33±3.38）、降低肝脂质过氧化（20.22±1.74）、增加膜结合酶（Na/KATPase：4.18±0.20；CaATPase：6.24±0.12）、增加抗氧化剂参数（CAT：64.89±4.13；SOD：6.01±0.11；GPx：8.55±0.05）、改变促炎细胞因子的水平（TNF-α：90.15±5.77；NF-κB：173.29±7.26；IL-6：178.11±3.16；IL-1β：48.26±1.89）和组织病理学研究来证实。我们的研究结果成功地表明了银纳米粒子的生物合成，并表现出在预防和干预肝细胞癌方面的化学保护潜力。
