State Key Laboratory for Mechanical Behavior of Materials, School of Materials Science and Engineering, Xi'an Jiaotong University, Xi'an 710049, P R China.
Shaanxi Key Laboratory of Biomedical Metal Materials, Northwest Institute for Non-ferrous Metal Research, Xi'an 710016 , P R China.
ACS Appl Mater Interfaces. 2020 Oct 7;12(40):44433-44446. doi: 10.1021/acsami.0c10032. Epub 2020 Sep 25.
A two-step electrochemical surface treatment has been developed to modify the CP Ti surface on commercially pure titanium grade 2 (CP Ti): (1) anodic oxidation to form TiO nanotube precoatings loaded with silver (Ag) and (2) microarc oxidation (MAO) to produce a porous Ca-P-Ag coating in an electrolyte containing Ag, Ca, and P. One-step MAO in the same electrolyte has also been used to produce porous Ca-P-Ag coatings without anodic oxidation and preloaded Ag as a control. Surface morphologies and alloying chemistry of the two coatings were characterized by SEM, EDS, and XPS. Biocompatibility and antimicrobial properties have been evaluated by the MTT method and co-culture of , respectively. It is demonstrated that porous coatings with high Ag content can be achieved on the CP Ti by the two-step treatment. The optimized MAO voltage for excellent comprehensive properties of the coating is 350 V, in which a suitable chemical equilibrium between Ag, Ca, and P contents and a Ca/P ratio of 1.67 similar to HA can be obtained, and the Ag particles are in the size of less than 100 nm and embedded into the underneath of the coating surface. After being contacted with for 1 and 7 days, the average bactericidal rates were 99.53 and 89.27% and no cytotoxicity was detected. In comparison, the one-step MAO coatings contained less Ag, had a lower Ca/P ratio, and showed lower antimicrobial ability than the two-step treated samples.
两步电化学表面处理已被开发用于修饰商用纯钛 2 级（CP Ti）的 CP Ti 表面：（1）阳极氧化形成负载银（Ag）的 TiO 纳米管预涂层，以及（2）微弧氧化（MAO）在含有 Ag、Ca 和 P 的电解液中生成多孔 Ca-P-Ag 涂层。一步 MAO 在相同的电解液中也被用于生产无阳极氧化和预加载 Ag 的多孔 Ca-P-Ag 涂层作为对照。通过 SEM、EDS 和 XPS 对两种涂层的表面形貌和合金化学进行了表征。通过 MTT 法和共培养分别评估了生物相容性和抗菌性能。结果表明，两步处理可以在 CP Ti 上获得具有高 Ag 含量的多孔涂层。优化的 MAO 电压为 350 V，可获得涂层的优异综合性能，其中 Ag、Ca 和 P 含量之间以及 Ca/P 比为 1.67（类似于 HA）的化学平衡得以实现，Ag 颗粒的尺寸小于 100nm，并嵌入涂层表面的下方。与 接触 1 天和 7 天后，平均杀菌率分别为 99.53%和 89.27%，未检测到细胞毒性。相比之下，一步 MAO 涂层的 Ag 含量较少，Ca/P 比较低，抗菌能力低于两步处理的样品。
