Campus Glória - Bloco 1CCG SALA 211A, BR-050, KM 78, Uberlândia, Minas Gerais 38410-337, Brazil; Graduate Program in Veterinary Science (PPGCVET), Universidade Federal de Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil.
Campus Glória - Bloco 1CCG SALA 211A, BR-050, KM 78, Uberlândia, Minas Gerais 38410-337, Brazil; College of Veterinary Medicine (FAMEV), Universidade Federal de Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil.
Domest Anim Endocrinol. 2021 Jan;74:106540. doi: 10.1016/j.domaniend.2020.106540. Epub 2020 Aug 17.
The aim of our study was to investigate the viability and validity of blood sampling from the upper lip mucosa in healthy dogs and cats for monitoring transoperative glycemia and compare the results with those obtained from samples taken from previously described blood sampling sites for determination of glycemia using a glucose meter. Blood glucose (BG) levels were determined in samples taken from the upper lip mucosa of 24 dogs and 31 cats undergoing neutering or spaying surgeries. These values were compared to those of samples obtained from other sites previously described for capillary blood glucose monitoring (marginal ear vein, carpal pad in dogs, metacarpal pad in cats) using a glucose meter. Additionally, BG from peripheral venous blood was determined using a glucose meter, and the gold standard enzymatic colorimetric assay. The clinical reliability of BG values taken from lip mucosa and from all the other BG values measured by the glucose meter was evaluated using the error grid analysis modified by Parkes et al (2000). The upper lip mucosa was an easily accessible site for the obtainment of appropriate blood samples, and glucose levels read in these samples correlated positively with glycemic values read in blood samples from all other sites in dogs and cats. All BG made using glucose meters taken from all sites were within the clinically acceptable range when compared with enzymatic colorimetric assay (gold standard), and were analytically accurate according to the error grid (zones A and B). All blood sampling sites described in this work can be used to assess transoperative glycemia. The upper lip mucosa is a viable blood sampling site for precise monitoring of transoperative glycemia in healthy dogs and cats and shows promise for alternative blood glucose monitoring.
我们研究的目的是调查在上唇黏膜采集健康犬猫血液样本以监测术中血糖的可行性和有效性，并将结果与使用血糖仪从先前描述的采血部位获得的血糖结果进行比较。我们对 24 只接受绝育手术的犬和 31 只接受绝育手术的猫的上唇黏膜样本进行了血糖（BG）水平的测定。这些值与使用血糖仪从先前描述的其他部位（边缘耳静脉、犬的腕垫、猫的掌垫）获得的毛细血管血糖监测样本的血糖值进行了比较。此外，还使用血糖仪和酶比色法测定了外周静脉血的 BG。采用 Parkes 等人（2000 年）修改的误差网格分析评估了从唇黏膜和血糖仪测量的所有其他 BG 值的临床可靠性。上唇黏膜是获取适当血液样本的容易接近部位，这些样本中的葡萄糖水平与犬和猫所有其他部位的血糖值呈正相关。与酶比色法（金标准）相比，所有使用血糖仪从所有部位获得的 BG 均在临床可接受范围内，并且根据误差网格（A 和 B 区）具有分析准确性。本研究中描述的所有采血部位均可用于评估术中血糖。在上唇黏膜处采血是一种可行的方法，可用于精确监测健康犬猫的术中血糖，并且有望替代血糖监测。
