Zeugswetter F K, Karlovitz S
Florian K. Zeugswetter, Klinik für Kleintiere, Department für Kleintiere und Pferde, Veterinärmedizinische Universität Wien, Veterinärplatz 1, A-1210 Wien, E-Mail:
Tierarztl Prax Ausg K Kleintiere Heimtiere. 2014;42(3):174-9.
Glucose measurements at the pinna or paws are an important part of the management of diabetic dogs. Despite extensive rubbing or warming of the testing site, an adequate blood sample cannot always be obtained. Therefore, the objective of this study was to evaluate the lip as an alternative sampling site with respect to practicability and tolerance by the dogs and reliability of the measured values.
Nine clinic-owned male-castrated healthy beagles were included in this prospective study. Glucose measurements were performed using a glucometer developed for veterinary use, before and 15 minutes after oral application of glucose syrup. Capillary blood was collected using two commercially available safety lancets (Wellion Safety lancet [23 gauge] and Wellion Safety lancet special [blade 0.8 mm]) at the buccal mucosa of the upper lip and at the pinna. Measured values were compared with those obtained from venous plasma (reference method).
Buccal glucose measurements were easy to perform and were well tolerated by the dogs without obvious pain reactions. There was no difference between the glucose concentrations from the lip samples and those determined using the reference method (p = 0.793, F < 1). The glucose application had no effect on the observed differences (reference value minus glucometer value; p = 0.63, F < 1). However, in one dog, a clinically problematic discrepancy of 3.6 mmol/l was observed. The special lancet caused noticeable secondary bleeding in two dogs.
The results of this study indicate that the buccal mucosa is a convenient and reliable alternative sampling site for glucose measurements in dogs. Problematic deviations from reference values are possible after oral glucose application and the use of blade-like needles is not encouraged.
测量耳廓或爪子处的血糖是糖尿病犬管理的重要组成部分。尽管对测试部位进行了大量摩擦或加热，但并非总能获得足够的血样。因此，本研究的目的是评估唇部作为替代采样部位在实用性、犬的耐受性以及测量值可靠性方面的情况。
本前瞻性研究纳入了9只临床所有的雄性去势健康比格犬。在口服葡萄糖糖浆之前和之后15分钟，使用专为兽医设计的血糖仪进行血糖测量。使用两种市售安全采血针（Wellion安全采血针[23号]和Wellion特殊安全采血针[刀片0.8毫米]）在上唇颊黏膜和耳廓处采集毛细血管血。将测量值与静脉血浆获得的值（参考方法）进行比较。
颊部血糖测量操作简便，犬耐受性良好，无明显疼痛反应。唇部样本的葡萄糖浓度与使用参考方法测定的浓度之间无差异（p = 0.793，F < 1）。葡萄糖应用对观察到的差异无影响（参考值减去血糖仪值；p = 0.63，F < 1）。然而，在一只犬中，观察到临床上有问题的3.6 mmol/l的差异。特殊采血针在两只犬中引起了明显的继发性出血。
本研究结果表明，颊黏膜是犬血糖测量方便且可靠的替代采样部位。口服葡萄糖后可能出现与参考值有问题的偏差，不鼓励使用刀片状针头。
