Jain Surbhi, Paliwal Ayushi, Gupta Vinay, Tomar Monika
Department of Physics and Astrophysics, University of Delhi, Delhi, 110007, India.
Department of Physics, Miranda House, University of Delhi, Delhi, 110007, India.
Biosens Bioelectron. 2020 Nov 15;168:112508. doi: 10.1016/j.bios.2020.112508. Epub 2020 Aug 20.
A novel, highly sensitive and low cost Long Range Surface Plasmon Resonance (LRSPR) biosensor for detecting uric acid, as a model analyte, has been developed in this work. Silicon dioxide (SiO) having low and tunable refractive index has been chosen as the dielectric layer for the excitation of LRSP modes replacing the most explored Cytop and Teflon polymers. The prepared LRSPR based uric acid bio-sensor gives good response characteristics with a high sensitivity of about 21.6°/mM and low limit of detection (LOD) of 0.02 mM. The fabricated LRSPR sensor was also evaluated to detect uric acid in real serum samples. The results yield a great scope to promote the development of robust, efficient and highly selective LRSPR based biosensors with SiO as tunable dielectric layer.
在本研究中，开发了一种新型、高灵敏度且低成本的用于检测尿酸（作为模型分析物）的长程表面等离子体共振（LRSPR）生物传感器。具有低且可调折射率的二氧化硅（SiO₂）已被选为用于激发LRSP模式的介电层，取代了研究最多的 Cytop 和特氟龙聚合物。所制备的基于LRSPR的尿酸生物传感器具有良好的响应特性，灵敏度高达约21.6°/mM，检测限（LOD）低至0.02 mM。所制造的LRSPR传感器还用于评估实际血清样品中的尿酸检测。这些结果为促进以SiO₂作为可调介电层的坚固、高效且高选择性的基于LRSPR的生物传感器的发展提供了广阔空间。
