Department of Parasitology, Chung Shan Medical University, 110, Section 1, Chien-Kuo North Road, Taichung 402, Taiwan.
Clinical Laboratory, Chung Shan Medical University Hospital, 110, Section 1, Chien-Kuo North Road, Taichung 402, Taiwan.
J Parasitol. 2020 Oct 1;106(5):564-571. doi: 10.1645/18-184.
Peroxisome proliferator-activated receptor gamma (PPARγ) regulates neuroinflammation, and its agonists act as neuroprotective agents. This study aims to investigate the correlation between PPARγ and proinflammatory enzyme expression in astroglia infected with Toxoplasma gondii tachyzoite in vitro. Our results showed that matrix metalloprotease (MMP)-2, MMP-9, cyclooxygenase-2 (COX-2), prostaglandin (PGE)-2, inducible nitric-oxide synthase (iNOS), and nitric oxide (NO) were significantly increased in T. gondii-infected astroglia. Furthermore, the expression levels of MMP-2, MMP-9, COX-2, PGE-2, iNOS, and NO were significantly decreased by rosiglitazone-a PPARγ agonist. By contrast, the treatment with GW9662, a PPARγ antagonist, efficiently increased the expression levels of MMP-2, MMP-9, COX-2, PGE-2, iNOS, and NO. These results suggested that the treatment with rosiglitazone offers a potential strategy for controlling the inflammatory factors in T. gondii infection.
过氧化物酶体增殖物激活受体 γ（PPARγ）调节神经炎症，其激动剂可作为神经保护剂。本研究旨在探讨体外感染弓形虫速殖子的星形胶质细胞中 PPARγ 与促炎酶表达之间的相关性。结果显示，弓形虫感染的星形胶质细胞中基质金属蛋白酶（MMP）-2、MMP-9、环氧化酶-2（COX-2）、前列腺素（PGE）-2、诱导型一氧化氮合酶（iNOS）和一氧化氮（NO）的表达显著增加。此外，PPARγ 激动剂罗格列酮可显著降低 MMP-2、MMP-9、COX-2、PGE-2、iNOS 和 NO 的表达水平。相反，PPARγ 拮抗剂 GW9662 可有效增加 MMP-2、MMP-9、COX-2、PGE-2、iNOS 和 NO 的表达水平。这些结果表明，罗格列酮的治疗为控制弓形虫感染中的炎症因子提供了一种潜在策略。
