Chair and Department of Conservative Dentistry with Endodontics, Medical University of Lublin, 20-059 Lublin, Poland.
Department of Otolaryngology, Masovian Specialist Hospital, 26-617 Radom, Poland.
Viruses. 2020 Sep 9;12(9):1008. doi: 10.3390/v12091008.
Recent reports have pointed to the link between persistent inflammation, oxidative stress, and carcinogenesis; however most of the studies concerning the role of viruses in head and neck cancer (HNC) are focused mainly on one type of virus. Our present study aimed to study the relationship between Epstein-Barr virus/human papilloma virus (EBV/HPV) coinfection and glutathione peroxidase (GPx) and superoxide dismutase (SOD) level in oropharyngeal cancer. Fresh-frozen tumor tissue samples were collected from 128 patients with oropharyngeal cancer infected with EBV or HPV or with EBV/HPV coinfection. After DNA extraction, EBV and HPV DNA was detected using a polymerase chain reaction (PCR) assay. GPx and SOD activity was determined in homogenates of cancer tissue using diagnostic kits produced by Randox Laboratories. Both GPx and SOD activity was statistically lower in patients with EBV/HPV coinfection than in a single EBV or HPV infection. Analysis of GPx and SOD activity in relation to histological grading and tumor, node (TN) classification revealed that in poorly-differentiated tumors, the level of antioxidant enzymes was lower compared with well-differentiated lesions and in cases with greater tumor dimensions and lymph-node involvement, both GPx and SOD activity was decreased. Further studies are necessary to clarify the influence of interplay between EBV, HPV, and oxidative stress on malignant transformation of upper aerodigestive tract epithelial cells.
最近的报告指出了持续炎症、氧化应激和致癌作用之间的联系；然而，大多数关于病毒在头颈部癌症（HNC）中作用的研究主要集中在一种类型的病毒上。本研究旨在研究 Epstein-Barr 病毒/人乳头瘤病毒（EBV/HPV）合并感染与口咽癌中谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）水平之间的关系。从 128 例感染 EBV 或 HPV 或 EBV/HPV 合并感染的口咽癌患者中采集新鲜冷冻肿瘤组织样本。提取 DNA 后，使用聚合酶链反应（PCR）检测 EBV 和 HPV DNA。使用 Randox 实验室生产的诊断试剂盒测定癌症组织匀浆中的 GPx 和 SOD 活性。与单一 EBV 或 HPV 感染相比，EBV/HPV 合并感染患者的 GPx 和 SOD 活性均统计学降低。对 GPx 和 SOD 活性与组织学分级和肿瘤、淋巴结（TN）分类的关系进行分析表明，在低分化肿瘤中，抗氧化酶水平低于高分化病变，在肿瘤尺寸较大和淋巴结受累的情况下，GPx 和 SOD 活性均降低。需要进一步研究以阐明 EBV、HPV 和氧化应激之间相互作用对上呼吸道上皮细胞恶性转化的影响。
