Pragnell I B, Wright E G, Lorimore S A, Adam J, Rosendaal M, DeLamarter J F, Freshney M, Eckmann L, Sproul A, Wilkie N
Beatson Institute for Cancer Research, Wolfson Laboratory, Glasgow, U.K.
Blood. 1988 Jul;72(1):196-201.
Spleen colony formation after transplantation of bone marrow cells into irradiated mice has been used as an assay for hematopoietic stem cells (CFU-S), but has serious limitations intrinsic to an in vivo assay. In this report we describe experiments using an in vitro clonogenic assay that is especially suitable for studies of stem cell regulation as defined growth factors and normal untreated bone marrow can be used. We have demonstrated that the colony-forming cells have proliferative properties in common with CFU-S and respond to specific proliferation regulators previously detected using the spleen colony assay.
将骨髓细胞移植到受照射小鼠体内后形成脾集落，已被用作造血干细胞（CFU-S）的一种检测方法，但作为一种体内检测方法存在严重的内在局限性。在本报告中，我们描述了使用一种体外克隆形成检测方法的实验，该方法特别适用于研究干细胞调节，因为可以使用确定的生长因子和正常未处理的骨髓。我们已经证明，集落形成细胞具有与CFU-S共同的增殖特性，并对先前使用脾集落检测法检测到的特定增殖调节因子有反应。
