Lorimore S A, Pragnell I B, Eckmann L, Wright E G
LRF Laboratory, MRC Radiobiology Unit, Chilton, U.K.
Leuk Res. 1990;14(5):481-9. doi: 10.1016/0145-2126(90)90036-9.
A clonogenic assay for cells that give rise to macroscopic colonies in agar or methyl cellulose cultures using untreated, normal murine bone marrow as a source of stem cells is described. We have characterized the clonogenic cell, which has been designated CFU-A, by comparing its properties with those of multipotential stem cells (assayed as CFU-S) and lineage-restricted progenitor cells (assayed as GM-CFC). The investigations have included assessment of proliferative status and response to CFU-S proliferation regulators, response to 5-fluorouracil, buoyant cell density, radial distribution in the femur and response to ionizing radiation. We conclude that the CFU-A has properties in common with CFU-S that differ from those of GM-CFC. The data are consistent with the CFU-A assay detecting part of the multipotential stem cell population also detected by spleen colony formation.
本文描述了一种克隆形成试验，该试验用于检测在琼脂或甲基纤维素培养物中能够形成宏观集落的细胞，其使用未经处理的正常小鼠骨髓作为干细胞来源。我们通过将已命名为CFU-A的克隆形成细胞的特性与多能干细胞（以CFU-S检测）和谱系受限祖细胞（以GM-CFC检测）的特性进行比较，对其进行了表征。研究内容包括评估增殖状态、对CFU-S增殖调节剂的反应、对5-氟尿嘧啶的反应、浮力细胞密度、在股骨中的径向分布以及对电离辐射的反应。我们得出结论，CFU-A具有与CFU-S相同的特性，这些特性不同于GM-CFC的特性。这些数据与CFU-A试验检测到的多能干细胞群体的一部分也可通过脾集落形成检测到这一结论一致。
