Departments of Radiology, Stony Brook Medicine, Stony Brook, NY 11794, United States.
Departments of Neurology, Stony Brook Medicine, Stony Brook, NY 11794, United States.
Brain Res. 2020 Dec 1;1748:147122. doi: 10.1016/j.brainres.2020.147122. Epub 2020 Sep 11.
Free radicals are downstream mediators of several cytotoxic cascades contributing to ischemic brain injury. Molecular hydrogen (H) is an antioxidant potentially useful in the treatment of stroke. Hydrogen is easy to deliver, biologically non-toxic and diffuses freely through all biological structures including the blood-brain barrier and cellular membranes. This study evaluated the efficacy of hydrogen treatments in a rat stroke model compared to vehicle-treated controls using multiparametric MRI and neurological tests. Additionally, comparison of H treatment alone was made with H combined with minocycline (H2M) treatment (12 rats per group). The primary findings were: i) H therapy reduced infarct volume in both H and H2M groups compared to controls at 1 and 7 days after stroke, and ii) both H and H2M improved neurologic functional recovery on day 7. The secondary outcomes were: iii) H2M treatment attenuated post-stroke hyperperfusion in the hyperacute phase, and iv) H2M markedly minimized white matter injury. In conclusion, this is the first study to use MRI to longitudinally study H and H2M treatment on ischemic stroke and the first study to compare H treatment combined with another potential stroke therapeutic (H2M).
自由基是导致缺血性脑损伤的几种细胞毒性级联反应的下游介质。氢气（H）是一种抗氧化剂，在中风治疗中可能有用。氢气易于输送，生物毒性低，可自由扩散通过所有生物结构，包括血脑屏障和细胞膜。本研究通过多参数 MRI 和神经学测试，比较了氢气治疗与载体治疗对照在大鼠中风模型中的疗效。此外，还比较了 H 单独治疗与 H 与米诺环素联合治疗（每组 12 只大鼠）。主要发现有：i）与对照组相比，H 和 H2M 治疗组在中风后 1 天和 7 天，H 治疗可降低梗死体积，ii）H 和 H2M 治疗均能改善第 7 天的神经功能恢复。次要结果有：iii）H2M 治疗可减轻超急性期的卒中后过度灌注，iv）H2M 可显著减轻白质损伤。总之，这是第一项使用 MRI 对缺血性中风的 H 和 H2M 治疗进行纵向研究的研究，也是第一项比较 H 治疗与另一种潜在的中风治疗药物（H2M）联合治疗的研究。
