Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheonju-si, Chungcheongbuk-do, 28116, Republic of Korea.
National Institute of Biological Resources, Environmental Research Complex, Gyoungseo-dong, Seo-gu, Incheon, 22689, Republic of Korea.
J Ethnopharmacol. 2021 Jan 30;265:113386. doi: 10.1016/j.jep.2020.113386. Epub 2020 Sep 10.
Daphne pseudomezereum var. koreana Hamaya is distributed in the Gangwon-do of South Korea and is traditionally used to treat chronic inflammatory diseases, including rheumatoid arthritis.
We investigated the anti-inflammatory effect of biflavonoid-rich fraction (BF) obtained from an extract of D. pseudomezereum leaves on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and mouse model of ovalbumin (OVA)-induced allergic asthma.
Neochamaejasmin B (NB) and chamaejasmin D (CD) were spectroscopically characterized as major components of BF obtained from the leaves of D. pseudomezereum. RAW264.7 cells pretreated with NB, CD and BF and activated by LPS (500 ng/ml) were used to assess the anti-inflammatory effects of these materials in vitro. To evaluate the protective effect of BF on allergic asthma, female BALB/c mice were sensitized to OVA by intraperitoneal (i.p.) injection and treated with BF by oral administration (15 or 30 mg/kg).
Pretreatment with BF inhibited LPS-stimulated nitric oxide (NO), TNF-α and IL-6, and led to upregulation of heme oxygenase-1 (HO-1) in RAW264.7 macrophages. Orally administered BF significantly inhibited the recruitment of eosinophils and the production of IL-5, IL-6, IL-13 and MCP-1 as judged by the analysis of BALF from OVA-induced asthma animal model. BF also decreased the levels of IgE in the serum of asthmatic mice. BF suppressed the influx of inflammatory cells into nearby airways and the hypersecretion of mucus by the airway epithelium of asthmatic mice. In addition, the increase in Penh in asthmatic mice was reduced by BF administration. Furthermore, BF led to Nrf2 activation and HO-1 induction in the lungs of mice.
These data have shown the anti-asthmatic effects of BF, and therefore we expect that BF may be a potential candidate as a natural drug/nutraceutical for the prevention and treatment of allergic asthma.
假瑞香（Daphne pseudomezereum var. koreana Hamaya）分布于韩国江原道，传统上用于治疗慢性炎症性疾病，包括类风湿性关节炎。
本研究旨在探讨从假瑞香（Daphne pseudomezereum）叶提取物中获得的富含双黄酮的馏分（BF）对脂多糖（LPS）刺激的 RAW264.7 巨噬细胞和卵清蛋白（OVA）诱导的过敏性哮喘小鼠模型的抗炎作用。
采用光谱法对 BF 中的主要成分 neochamaejasmin B（NB）和 chamaejasmin D（CD）进行了表征。用 LPS（500ng/ml）激活 RAW264.7 细胞之前，用 NB、CD 和 BF 预处理这些细胞，以评估这些物质的抗炎作用。为了评估 BF 对过敏性哮喘的保护作用，雌性 BALB/c 小鼠通过腹腔（i.p.）注射致敏 OVA，并通过口服（15 或 30mg/kg）给予 BF 治疗。
BF 预处理抑制了 LPS 刺激的一氧化氮（NO）、TNF-α和 IL-6 的产生，并上调了 RAW264.7 巨噬细胞中的血红素加氧酶-1（HO-1）。口服 BF 可显著抑制 OVA 诱导的哮喘动物模型 BALF 中嗜酸性粒细胞的募集以及 IL-5、IL-6、IL-13 和 MCP-1 的产生。BF 还降低了哮喘小鼠血清中的 IgE 水平。BF 抑制了哮喘小鼠气道上皮细胞附近气道中炎症细胞的涌入和粘液的过度分泌。此外，BF 可降低哮喘小鼠的 Penh 值。此外，BF 导致小鼠肺部 Nrf2 激活和 HO-1 诱导。
这些数据表明 BF 具有抗哮喘作用，因此我们期望 BF 可能成为预防和治疗过敏性哮喘的天然药物/营养保健品的潜在候选药物。
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