中国新疆维吾尔族和汉族人群中血管紧张素基因M235T与T174M多态性和心房颤动的关系

Relationship between M235T and T174M polymorphisms in angiotensin gene and atrial fibrillation in Uyghur and Han populations of Xinjiang, China.

作者信息

Kuken Bannu, Yang Yuchun, Liu Zhiqiang, He Pengyi, Wulasihan Muhuyati

机构信息

Department of Cardiology, The Second Affiliated Hospital of Xinjiang Medical University Urumqi, Xinjiang Uygur Autonomous Region, China.

Department of Cardiology, The First Affiliated Hospital of Xinjiang Medical University Urumqi, Xinjiang Uygur Autonomous Region, China.

出版信息

Int J Clin Exp Pathol. 2020 Aug 1;13(8):2065-2074. eCollection 2020.

PMID:32922602

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7476944/

Abstract

OBJECTIVE

To elucidate the association between angiotensin gene (AGT) M235T and T174M genetic polymorphisms in Han and Uyghur patients with atrial fibrillation (AF) in Xinjiang, China.

METHODS

100 cases of patients with AF of Han and 100 cases of patients with AF of Uyghur were selected as the experimental group, and 100 cases of patients with non-AF of Uyghur and non-AF of Han were selected as the control group. We amplified the AGT M235T site and AGT T174M site by PCR in 4 groups of patients, verified the polymorphism of the sites by gene sequencing, and compared their differences in each group.

RESULTS

The high risk factors for AF such as sex, left atrial diameter (LAD), right atrial diameter (RAD), and coronary heart disease were significantly different between the Han case group and the control group ( < 0.05). There were significant differences in age, LAD, RAD, coronary heart disease and smoking as contributors to AF between Uyghur case group and control group ( < 0.05). The genotype frequency distribution of AGT M235T and AGT T174M loci in the AF group and control group of Han and Uyghur was in accordance with Hardy-Weinberg equilibrium law test. There was a significant difference in genotype frequency and allele frequency of AGT M235T locus between Han group, Uyghur AF group and control group ( < 0.05).

CONCLUSION

The AGT M235T and AGT T174M loci were associated with the occurrence of atrial fibrillation in the Han and Uyghur ethnic groups in Xinjiang.

摘要

目的

阐明中国新疆汉族和维吾尔族心房颤动（AF）患者血管紧张素基因（AGT）M235T和T174M基因多态性之间的关联。

方法

选取100例汉族AF患者和100例维吾尔族AF患者作为实验组，选取100例汉族非AF患者和100例维吾尔族非AF患者作为对照组。我们对4组患者通过聚合酶链反应（PCR）扩增AGT M235T位点和AGT T174M位点，通过基因测序验证位点的多态性，并比较各组之间的差异。

结果

汉族病例组与对照组之间，AF的高危因素如性别、左心房直径（LAD）、右心房直径（RAD）和冠心病有显著差异（<0.05）。维吾尔族病例组与对照组之间，年龄、LAD、RAD、冠心病和吸烟作为AF的促成因素有显著差异（<0.05）。汉族和维吾尔族AF组与对照组中AGT M235T和AGT T174M位点的基因型频率分布符合哈迪-温伯格平衡定律检验。汉族组、维吾尔族AF组和对照组之间AGT M235T位点的基因型频率和等位基因频率有显著差异（<0.05）。