Chen Xiuying, Liu Xiaolei, Zuo Xuzheng, Cheng Chang, Huang Wen, Xie Peng
Department of Neurology, Second Affiliated Hospital of Army Medical University Chongqing 400037, China.
Department of Neurology, The First Affiliated Hospital, Chongqing Medical University Chongqing 400016, China.
Int J Clin Exp Pathol. 2020 Aug 1;13(8):2082-2091. eCollection 2020.
to investigate the diagnostic value of circulating miR-134 in major depressive disorder (MDD). Plasma miR-134 levels were determined in patients with MDD, bipolar disorder (BD), and schizophrenia (SCZ), as well as in healthy controls using quantitative real-time PCR. The diagnostic value of circulating miR-134 was assessed using receiver-operator characteristic (ROC) curve analysis. A chronic unpredictable mild stress (CUMS) rat model was established to evaluate the miR-134 expression pattern in serum and diseased brain tissues. Plasma miR-134 levels in MDD patients were significantly lower than those in healthy controls and patients with BD or SCZ. In addition, plasma miR-134 levels could be used to discriminate MDD patients from healthy controls (areas under the curve (AUC) = 0.901), healthy controls + patients with BD or SCZ (AUC = 0.864), patients with SCZ (AUC = 0.856), patients with SCZ or BD (AUC = 0.828), and patients with BD (AUC = 0.799), respectively. In CUMS rats, the miR-134 levels in plasma (P < 0.001), prefrontal cortex (P = 0.031), and hippocampus (P = 0.006) were consistently reduced compared with those in control group, with the exception of those in olfactory bulb (P = 0.565). Circulating miR-134 a potential biomarker for MDD diagnosis and monitoring.
探讨循环miR-134在重度抑郁症（MDD）中的诊断价值。采用定量实时PCR法测定MDD患者、双相情感障碍（BD）患者、精神分裂症（SCZ）患者以及健康对照者血浆中的miR-134水平。使用受试者工作特征（ROC）曲线分析评估循环miR-134的诊断价值。建立慢性不可预测轻度应激（CUMS）大鼠模型，以评估血清和患病脑组织中miR-134的表达模式。MDD患者血浆miR-134水平显著低于健康对照者以及BD或SCZ患者。此外，血浆miR-134水平可用于区分MDD患者与健康对照者（曲线下面积（AUC）=0.901）、健康对照者+BD或SCZ患者（AUC = 0.864）、SCZ患者（AUC = 0.856）、SCZ或BD患者（AUC = 0.828）以及BD患者（AUC = 0.799）。在CUMS大鼠中，与对照组相比，血浆（P < 0.001）、前额叶皮质（P = 0.031）和海马体（P = 0.006）中的miR-134水平持续降低，但嗅球中的miR-134水平除外（P = 0.565）。循环miR-134是MDD诊断和监测的潜在生物标志物。
