Aminan Aimi Wahidah, Kit Leonard Lim Whye, Hui Chung Hung, Sulaiman Badiozaman
Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
Faculty of Industrial Science and Technology, Universiti Malaysia Pahang, 26600 Pekan, Pahang, Malaysia.
Trop Life Sci Res. 2020 Jul;31(2):33-49. doi: 10.21315/tlsr2020.31.2.3. Epub 2020 Aug 6.
The genus is one of the most species-rich genus among the freshwater fishes and cryptic diversity has been a major hindrance in species identification in the past four decades due to their high similarities in terms of morphology. This study aimed to investigate this issue both morphologically and molecularly. In this study, a total of 23 morphometric parameters were used to differentiate the 103 fish samples harvested from different regions of Sarawak state of Malaysia via Multivariate Stepwise Discriminant Function Analysis (SDFA). Then, () gene was utilised to further distinguish 33 of these fishes, followed by sequence and phylogenetic analysis. Our results unravelled pre-anal length as strongest morphometric discriminant (100%) and that all eight species tested are monophyletic except for and , revealing possible cryptic species. Further investigations are vital to enrich the data from this study for cryptic diversity and conservation studies in future.
该属是淡水鱼类中物种最为丰富的属之一，在过去的四十年里，由于其形态高度相似，隐秘多样性一直是物种鉴定的主要障碍。本研究旨在从形态学和分子学两方面对这一问题进行调查。在本研究中，共使用23个形态测量参数，通过多变量逐步判别函数分析（SDFA）来区分从马来西亚砂拉越州不同地区采集的103个鱼类样本。然后，利用（）基因进一步区分其中的33条鱼，随后进行序列和系统发育分析。我们的结果表明，肛前长度是最强的形态学判别指标（100%），除了（某两种鱼未明确写出）和（某两种鱼未明确写出）外，所有测试的8个（某属名未明确写出）物种都是单系的，这揭示了可能存在隐秘物种。进一步的调查对于丰富本研究的数据以用于未来的隐秘多样性和保护研究至关重要。
