Ashfaq Ahsan, Ejaz Ayesha, Ahmed Fareena Khalil, Un Nisa Noor, Langhani Suresh, Riffat Sumaira, Ariff Madiha
Physiology, Liaquat National Medical College, Karachi, PAK.
Nephrology, Jinnah Postgraduate Medical Centre, Karachi, PAK.
Cureus. 2020 Aug 10;12(8):e9636. doi: 10.7759/cureus.9636.
Objectives To measure serum neopterin levels in blood donors of the local population and to study its relationship with age and blood group of transfusion-transmitted infection (TTI) negative blood donors. Methodology This cross-sectional study was carried out in the Department of Physiology at Liaquat National Hospital and Medical College (LNMC), Karachi, Pakistan, in collaboration with the Basic Medical Sciences Institute and Jinnah Post Graduate Medical Centre (JPMC). Data were collected from January 2018 to July 2018. A total of 174 blood donors participated in the study, who were selected by using a random sampling technique. They were screened through the standard procedures used for screening at the JPMC blood bank for TTIs as per the World Health Organization recommendations. Serum neopterin was estimated using enzyme-linked immunosorbent assay (ELISA) kits. Data analysis was performed using Statistical Package for Social Sciences (SPSS) version 23 (IBM Corp., Armonk, NY, USA). Chi-square and ANOVA (analysis of variance) were applied, and tests of significance were kept as P < 0.05. Results Neopterin level in the serum of TTI-positive blood donors was 15.1 ± 4.59 nmol/L, which was above the normal range and cutoff value of 10 nmol/L, whereas the neopterin level in the serum of TTI-negative blood donors was 6.1 ± 1.82 nmol/L (P = 0.001). In TTI-negative blood donors, the neopterin levels were within normal limits and were not influenced by age and blood groups (P > 0.05). Conclusions Serum neopterin levels did not report any significant difference in terms of age and blood group of TTI-negative blood donors and were seen to be within normal limits.
目的 测量当地人群献血者的血清新蝶呤水平，并研究其与输血传播感染（TTI）阴性献血者的年龄及血型的关系。方法 本横断面研究在巴基斯坦卡拉奇利亚卡特国家医院和医学院（LNMC）生理学系开展，与基础医学科学研究所和真纳研究生医学中心（JPMC）合作。数据收集时间为2018年1月至2018年7月。共有174名献血者参与研究，采用随机抽样技术选取。按照世界卫生组织的建议，通过JPMC血库用于TTI筛查的标准程序对他们进行筛查。使用酶联免疫吸附测定（ELISA）试剂盒估算血清新蝶呤。使用社会科学统计软件包（SPSS）23版（美国纽约州阿蒙克市IBM公司）进行数据分析。应用卡方检验和方差分析（ANOVA），显著性检验设定为P<0.05。结果 TTI阳性献血者血清中的新蝶呤水平为15.1±4.59 nmol/L，高于正常范围和10 nmol/L的临界值，而TTI阴性献血者血清中的新蝶呤水平为6.1±1.82 nmol/L（P=0.001）。在TTI阴性献血者中，新蝶呤水平在正常范围内，不受年龄和血型影响（P>0.05）。结论 TTI阴性献血者的血清新蝶呤水平在年龄和血型方面未报告任何显著差异，且在正常范围内。
