Ali Mohamed A, Razafindralambo Hary L, Conti Giuseppina, De Coninck Joël
Physics of Surfaces and Interfaces Laboratory, University of Mons, 19, Avenue Maistriau, B-7000 Mons, Belgium.
Gembloux Agro-Bio Tech, TERRA Teaching and Research Centre, University of Liege, Avenue de la Faculté 2B, B140, B-5030 Gembloux, Belgium.
ACS Omega. 2020 Aug 28;5(35):22348-22355. doi: 10.1021/acsomega.0c02728. eCollection 2020 Sep 8.
Most probiotic-based products are available in powder particles under different solid-state forms. Such diversity can affect the probiotic stability, viability, and performance at different stages of processing, storage, and use. Here, we apply complementary physical chemistry techniques to characterize the bulk and surface properties of probiotic powder particles under different forms and report quantitative results of a highly concentrated multistrain reference product. The solid particle morphology, size/shape distribution, and the powder surface wettability in the compressed disc and porous packed bed forms are successively measured by sessile drop and capillary rise techniques. A complete wettability of the disc surface is observed through equilibrium contact angle measurements for various solvents, whereas the associated capillary rise data exhibit two regimes: a power law regime for the first few moments followed by a second regime, which can be described using Darcy's law. The use of this modeling approach shows the possibility of assessing the particle-packed bed permeability and porosity. These results open a new route of the structure-activity relationship study on the impact of probiotic solid particles on their functionalities and performance in promoting health benefits, related particularly to the human and animal gut permeability. This statement also strengthens the idea of using the compressed disc technique for easily performing probiotic wettability measurements.
大多数基于益生菌的产品以不同固态形式的粉末颗粒存在。这种多样性会影响益生菌在加工、储存和使用的不同阶段的稳定性、活力和性能。在此，我们应用互补的物理化学技术来表征不同形式下益生菌粉末颗粒的整体和表面性质，并报告一种高浓度多菌株参考产品的定量结果。通过静滴法和毛细管上升技术依次测量了压缩圆盘和多孔填充床形式下固体颗粒的形态、尺寸/形状分布以及粉末表面润湿性。通过对各种溶剂的平衡接触角测量观察到圆盘表面的完全润湿性，而相关的毛细管上升数据呈现出两种状态：最初几个时刻的幂律状态，随后是可以用达西定律描述的第二种状态。这种建模方法的应用表明了评估颗粒填充床渗透率和孔隙率的可能性。这些结果为益生菌固体颗粒对其促进健康益处的功能和性能影响的构效关系研究开辟了一条新途径，尤其与人和动物肠道通透性相关。这一表述也强化了使用压缩圆盘技术轻松进行益生菌润湿性测量的想法。
