Department of Gembloux Agro-Bio Tech, University of Liege, Avenue de la Faculté d'Agronomie 2B-BAT 140 TERRA, B-5030, Gembloux, Belgium.
Department of Biology and Biotechnology, "C. Darwin", Sapienza University of Rome, Rome, Italy.
Probiotics Antimicrob Proteins. 2020 Sep;12(3):1193-1202. doi: 10.1007/s12602-019-09590-1.
Variability in the efficacy, safety, and quality of probiotic formulations depends on many factors, including process conditions used by manufacturers. Developing reliable analytical tools is therefore essential to quickly monitor manufacturing differences in probiotic samples for their quality assessment. Here, multi-strain probiotics from two production sites and countries were investigated by proteomics and physico-chemistry approaches in relation to the protective effect on gut barrier. Proteomic analyses showed differences in protein abundances, identities, and origins of two series of VSL#3 samples from different sites. Even though both formulations were qualitatively similar in thermal and colloidal profiles, significant differences were quantitatively observed in terms of maximum decomposition temperature Tmax (p < 0.05) and phase transition temperature Tm (p < 0.01). Such variability in physical and biochemical features impacts on probiotic functionalities and translates into a differential modulation of gut permeability in mice. Physico-chemical scans provide coherent data with proteomics and represent a new tool for time and cost effective quality control of probiotic-based products.
益生菌制剂的功效、安全性和质量的变异性取决于许多因素，包括制造商使用的工艺条件。因此，开发可靠的分析工具对于快速监测益生菌样品的制造差异以进行质量评估至关重要。在这里，通过蛋白质组学和物理化学方法研究了来自两个生产地点和国家的多菌株益生菌与肠道屏障保护作用的关系。蛋白质组学分析显示，来自不同地点的两系列 VSL#3 样品的蛋白质丰度、身份和来源存在差异。尽管两种配方在热和胶体特性方面定性相似，但在最大分解温度 Tmax（p<0.05）和相变温度 Tm（p<0.01）方面观察到定量差异。物理和生化特性的这种可变性会影响益生菌的功能，并转化为对小鼠肠道通透性的差异调节。物理化学扫描与蛋白质组学提供一致的数据，代表了用于控制基于益生菌的产品的时间和成本有效的质量控制的新工具。
