Tavakkoli Hadi, Imani Masoud, Seyyed Mohammad Rahchamani, Rezvani Mohsen
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Department of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Int J Reprod Biomed. 2020 Aug 19;18(8):579-590. doi: 10.18502/ijrm.v13i8.7497. eCollection 2020 Aug.
Methenamine is a worldwide antibacterial agent for urinary system infections in human and animals. The effect of methenamine consumption during early phase of pregnancy is not fully clarified in previous studies. Vascular development is the essential part of the early embryonic growth.
In this study, we used chicken chorioallantoic membrane to evaluate the effects of methenamine administration on angiogenesis process as a model.
In this experimental study, 20 Ross 308 eggs (mean weight 55 4) were incubated. The eggs were divided into two equal groups (n = 10/each). In the first group, methenamine (150 mg/kg egg weight) was injected on the shell membrane, and in the second group (control group) phosphate-buffered salineas injected. Methenamine was inoculated at 96 and 120 hrafter incubation; 24 hrafter the last inoculation, the eggs were removed and the egg's shell was incised. Then, the development of vascular network and vascular endothelial growth factor Aexpression was evaluated.
Angiogenesis was significantly decreased after methenamine treatment. The indexes such as areas containing vessels, the vessels' length, the percentage of angiogenesis developing areas, and vascular complexity in the treatment group receiving methenamine were significantly reduced compared to the control group. expression was suppressed in the methenamine treated group.
According to the achieved results, it was defined that methenamine could have an inhibitory effect on the growth and development procedures of extraembryonic vasculature.
乌洛托品是一种用于人类和动物泌尿系统感染的全球通用抗菌剂。先前的研究尚未完全阐明孕期早期服用乌洛托品的影响。血管发育是早期胚胎生长的重要组成部分。
在本研究中，我们以鸡胚绒毛尿囊膜为模型评估乌洛托品给药对血管生成过程的影响。
在本实验研究中，孵化20枚罗斯308鸡蛋（平均重量55±4）。将鸡蛋分为两组，每组10枚。第一组在壳膜上注射乌洛托品（150毫克/千克蛋重），第二组（对照组）注射磷酸盐缓冲盐水。孵化后96小时和120小时接种乌洛托品；最后一次接种后24小时，取出鸡蛋并切开蛋壳。然后，评估血管网络的发育和血管内皮生长因子A的表达。
乌洛托品处理后血管生成显著减少。与对照组相比，接受乌洛托品治疗的组中含血管区域、血管长度、血管生成发育区域百分比和血管复杂性等指标均显著降低。乌洛托品处理组中血管内皮生长因子A的表达受到抑制。
根据所得结果，确定乌洛托品可能对胚外血管系统的生长和发育过程具有抑制作用。
