Majidian Eydgahi Shokat, Baharara Javad, Zafar Balanezhad Saeideh, Asadi Samani Majid
Research Center for Animal Development, Applied Biology & Biology Department, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Student's Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Avicenna J Phytomed. 2015 May-Jun;5(3):174-81.
Much attention is paid to angiogenesis due to its mutual role in health and disease. Therefore, the effect of various chemical and physical agents on inhibition of this process has been recently studied. This study was conducted to investigate the synergic effect of glycyrrhizic acid and electromagnetic field on angiogenesis.
In this experimental study, 44 Ross fertilized chicken eggs were randomly divided into four groups, one control and three experimental. Control group was kept with dimethyl sulfoxide on the eighth day, experimental group 1 treated with 200 gauss, 50 Hz electromagnetic field on the 10th day, experimental group 2 treated with 1 mg/ml glycyrrhizic acid on the eighth day, and experimental group 3 simultaneously treated with glycyrrhizic acid on the eighth day and electromagnetic field on the 10th day. On the 12th day, the images of chorioallantoic membrane samples were prepared using photostreomicroscope and the number and length of vessels were measured.
The mean number of vessels in the experimental groups 1 and 3 (29.31±3.60 and 27.43±4.61, respectively) was not significantly different from that in the control group (29.11±4.76) (p>0.05). The length of vessels in the experimental groups 1 and 3 (52.35±3.25 mm and 54.94±4.70 mm, respectively) decreased significantly (p<0.05) compared with the control group (61.79±6.46 mm). In experimental group 2, both length and number of vessels (54.53±5.85 mm and 23.96±3.94) decreased significantly compared with the control group (p<0.05).
Electromagnetic field and glycyrrhizic acid separately led to inhibition of angiogenesis. However, use of electromagnetic field accompanied with glycyrrhizic acid not only did not increase but also decreased the inhibitory effect.
由于血管生成在健康和疾病中都起着重要作用，因此备受关注。因此，最近研究了各种化学和物理因素对这一过程的抑制作用。本研究旨在探讨甘草酸和电磁场对血管生成的协同作用。
在本实验研究中，44枚罗斯受精鸡蛋被随机分为四组，一组为对照组，三组为实验组。对照组在第8天用二甲基亚砜处理，实验组1在第10天用200高斯、50赫兹的电磁场处理，实验组2在第8天用1毫克/毫升的甘草酸处理，实验组3在第8天用甘草酸处理，第10天用电磁场处理。在第12天，使用光显微镜制备绒毛尿囊膜样本的图像，并测量血管的数量和长度。
实验组1和实验组3的血管平均数量（分别为29.31±3.60和27.43±4.61）与对照组（29.11±4.76）相比无显著差异（p>0.05）。实验组1和实验组3的血管长度（分别为52.35±3.25毫米和54.94±4.70毫米）与对照组（61.79±6.46毫米）相比显著降低（p<0.05）。在实验组2中，血管的长度和数量（54.53±5.85毫米和23.96±3.94）与对照组相比均显著降低（p<0.05）。
电磁场和甘草酸单独使用均可抑制血管生成。然而，电磁场与甘草酸联合使用不仅没有增强，反而降低了抑制效果。
