Ogunlade Babatunde, Adelakun Sunday, Iteire Kingsley
Department of Human Anatomy, Federal University of Technology, Akure, Ondo State, Nigeria.
Department of Human Anatomy, University of Medical Sciences, Ondo city, Ondo State, Nigeria.
Int J Reprod Biomed. 2020 Aug 19;18(8):611-624. doi: 10.18502/ijrm.v13i8.7503. eCollection 2020 Aug.
The exposure of male individual to environmental toxicant is regarded as a channel that results in reduced sperm counts and infertility.
This study investigated the ameliorative response of Sulforaphane (SFN) on Aluminum trichloride (AlCl ) induced testicular toxicity in adult male Wistar rats.
A total of 32 adult male Wistar rats (180-200 gm between 8-10 wk) were divided into four groups (n = 8/each). Group A) received distilled water orally as placebo; Group B) received 100 mg/kgbw AlCl only orally; Group C) received 100 mg/kgbw AlCl and 100 mg/kgbw SFN orally; and Group D) received 100 mg/kgbw SFN only orally. After 28 days of experiment, animals underwent cervical dislocation, blood serum was obtained for analysis, and testes were harvested for biochemical assays, histology, hormonal profile, and sperm characterization.
The sperm parameters showed a significant difference within the AlCl only group compared with the control and SFN only groups (p = 0.02). However, AlCl and SFN co-treatment showed improvement in the motility, viability, and sperm count compared with the AlCl only group (p = 0.02). Furthermore, there was a significant decline in the levels of hormones profile and antioxidant status in AlCl only group compared to the control and SFN only (p = 0.02). The testicular histoarchitecture of the AlCl only group showed shrinkage of seminiferous tubules, spermatogenesis disruption, and empty lumen compared to the control and SFN only groups.
The present study revealed the ameliorative response of SFN on AlCl -induced testicular toxicity on serum hormone profiles, antioxidant status, lipid peroxidation, and histomorphometric analysis through oxidative stress.
男性个体暴露于环境毒物被视为导致精子数量减少和不育的一个途径。
本研究调查了萝卜硫素（SFN）对三氯化铝（AlCl₃）诱导的成年雄性Wistar大鼠睾丸毒性的改善作用。
将32只成年雄性Wistar大鼠（8 - 10周龄，体重180 - 200克）分为四组（每组n = 8）。A组口服蒸馏水作为安慰剂；B组仅口服100 mg/kgbw的AlCl₃；C组口服100 mg/kgbw的AlCl₃和100 mg/kgbw的SFN；D组仅口服100 mg/kgbw的SFN。实验28天后，对动物进行颈椎脱臼处死，采集血清进行分析，并摘取睾丸进行生化检测、组织学检查、激素水平测定和精子特征分析。
仅AlCl₃组的精子参数与对照组和仅SFN组相比有显著差异（p = 0.02）。然而，AlCl₃和SFN联合处理组与仅AlCl₃组相比，精子活力、存活率和精子数量有所改善（p = 0.02）。此外，仅AlCl₃组的激素水平和抗氧化状态与对照组和仅SFN组相比显著下降（p = 0.02）。与对照组和仅SFN组相比，仅AlCl₃组的睾丸组织架构显示生精小管萎缩、精子发生破坏和管腔空虚。
本研究揭示了SFN对AlCl₃诱导的睾丸毒性在血清激素水平、抗氧化状态、脂质过氧化和通过氧化应激进行的组织形态计量分析方面具有改善作用。
