Adelakun S A, Akintunde O W, Ogunlade B, Adeyeluwa B E
Department of Human Anatomy, College of Health Sciences, Federal University of Technology, Akure, Nigeria; Department of Anatomy, College of Health Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Department of Anatomy, College of Health Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Morphologie. 2023 Mar;107(356):80-98. doi: 10.1016/j.morpho.2022.05.002. Epub 2022 May 31.
This study examine the histochemical and histomorphological effect of 1-isothiocyanato-4-methyl sulfonyl butane (SFN) on cisplatin (CP) induced testicular alteration and cholesterol homeostasis.
Ninety adult-male Sprague-Dawley rats were randomized into nine groups of ten (n=10) rats each. Group A (control) received normal saline, group B received a single dose of 10mg/Kg body weight (bwt) CP (i.p.), group C received 50mg/Kg bwt of SFN, group D received 100mg/Kg bwt of SFN, group E received 10mg/Kg bwt CP and 50mg/Kg bwt of SFN, group F received 10mg/Kg bwt CP and 100mg/Kg bwt of SFN, group G received 10mg/Kg bwt CP and 50mg/Kg bwt vitamin C, group H received 50mg/Kg bwt of SFN and 10mg/Kg bwt CP, group I received 100mg/Kg bwt of SFN and 10mg/Kg bwt CP. The procedure lasted for 56 days. Testicular histomorphology and histochemistry, testicular testosterone, sperm parameters, total antioxidant status (TSA), total oxidant status (TOS), oxidative stress index (OSI), and serum lipid profile were examined.
Cisplatin decrease intra-testicular testosterone, sperm quality, and expression of glycogen and increases testicular TOS and OSI, serum lipid profile, collagen, and disruption of germinal epithelium. However, the intervention of SFN reversed the effect of CP on testes' weight and volume, DSP, ESP, testosterone production, TAS, TOS, and OSI. Histoarchitectecture showing normal seminiferous tubules and even distribution of glycogen and collagen fibers.
Treatment with SFN ameliorate CP-induced testicular toxicity by reversing the cytotoxic mechanisms of CP.
本研究考察了1-异硫氰酸基-4-甲磺酰基丁烷（SFN）对顺铂（CP）诱导的睾丸改变及胆固醇稳态的组织化学和组织形态学影响。
将90只成年雄性Sprague-Dawley大鼠随机分为9组，每组10只（n = 10）。A组（对照组）给予生理盐水，B组给予单剂量10mg/kg体重（bwt）的CP（腹腔注射），C组给予50mg/kg bwt的SFN，D组给予100mg/kg bwt的SFN，E组给予10mg/kg bwt CP和50mg/kg bwt的SFN，F组给予10mg/kg bwt CP和100mg/kg bwt的SFN，G组给予10mg/kg bwt CP和50mg/kg bwt维生素C，H组给予50mg/kg bwt的SFN和10mg/kg bwt CP，I组给予100mg/kg bwt的SFN和10mg/kg bwt CP。该过程持续56天。检测睾丸组织形态学和组织化学、睾丸睾酮、精子参数、总抗氧化状态（TSA）、总氧化状态（TOS）、氧化应激指数（OSI）以及血清脂质谱。
顺铂降低了睾丸内睾酮、精子质量和糖原表达，并增加了睾丸TOS和OSI、血清脂质谱、胶原蛋白以及生精上皮的破坏。然而，SFN的干预逆转了CP对睾丸重量和体积、DSP、ESP、睾酮生成、TAS、TOS和OSI的影响。组织架构显示生精小管正常，糖原和胶原纤维分布均匀。
SFN治疗通过逆转CP的细胞毒性机制改善了CP诱导的睾丸毒性。
