Stephenson Makeda, Reich Daniel H, Boheler Kenneth R
Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, Maryland, USA.
Department of Physics and Astronomy, The Johns Hopkins University, Baltimore, Maryland, USA.
Vasc Biol. 2019 Dec 12;2(1):R1-R15. doi: 10.1530/VB-19-0028. eCollection 2020.
The reproducible generation of human-induced pluripotent stem cell (hiPSC)-derived vascular smooth muscle cells (vSMCs) has been critical to overcoming many limitations of animal and primary cell models of vascular biology and disease. Since this initial advance, research in the field has turned toward recapitulating the naturally occurring subtype specificity found in vSMCs throughout the body, and honing functional models of vascular disease. In this review, we summarize vSMC derivation approaches, including current phenotype and developmental origin-specific methods, and applications of vSMCs in functional disease models and engineered tissues. Further, we discuss the challenges of heterogeneity in hiPSC-derived tissues and propose approaches to identify and isolate vSMC subtype populations.
可重复生成人诱导多能干细胞（hiPSC）来源的血管平滑肌细胞（vSMC）对于克服血管生物学和疾病动物模型及原代细胞模型的诸多局限性至关重要。自这一初步进展以来，该领域的研究已转向重现全身vSMC中自然存在的亚型特异性，并完善血管疾病的功能模型。在本综述中，我们总结了vSMC的衍生方法，包括当前基于表型和发育起源特异性的方法，以及vSMC在功能性疾病模型和工程组织中的应用。此外，我们讨论了hiPSC衍生组织中异质性的挑战，并提出了识别和分离vSMC亚型群体的方法。
