盐酸5-氨基乙酰丙酸负载微泡介导的胰腺癌细胞声动力治疗
5-Aminolevulinic acid hydrochloride loaded microbubbles-mediated sonodynamic therapy in pancreatic cancer cells.
作者信息
Yang Wei, Xu Haitao, Liu Qinghao, Liu Chunxun, Hu Jiahe, Liu Ping, Fang Tianyi, Bai Yuxian, Zhu Jiuxin, Xie Rui
机构信息
Harbin Medical University Cancer Hospital, Harbin, China.
Department of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratories of Cardiovascular Medicine Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, China.
出版信息
Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):1178-1188. doi: 10.1080/21691401.2020.1813743.
5-Aminolevulinic acid hydrochloride (ALA)-mediated sonodynamic therapy (SDT) had anti-tumour effect on pancreatic cancer cells. Hence, ALA loaded lipid/poly(lactic-co-glycolic acid) (PLGA) microbubbles (MBs)-mediated SDT for pancreatic cancer has great potential. The average size of ALA-lipid MBs and ALA-PLGA MBs was about 3.0 µm. The two kinds of MBs had good biocompatibility to normal HPDE6-C7 cells and were not toxic to pancreatic cancer cells. Compared with ALA-induced SDT, a statistically significant decrease in cell viability was observed in ALA lipid/PLGA MBs combined with ultrasound groups in AsPC-1 and BxPC-3 cells ( < .05). Obvious effect on the apoptotic rate, apoptosis and pyroptosis morphology, enhanced reactive oxygen species was found in ALA-lipid/PLGA MBs mediated SDT . Through study, we found ALA-lipid/PLGA MBs-mediated SDT was a promise treatment for pancreatic cancer.
5-氨基乙酰丙酸盐酸盐（ALA）介导的声动力疗法（SDT）对胰腺癌细胞具有抗肿瘤作用。因此，负载ALA的脂质/聚乳酸-羟基乙酸共聚物（PLGA）微泡（MBs）介导的胰腺癌SDT具有巨大潜力。ALA-脂质微泡和ALA-PLGA微泡的平均尺寸约为3.0μm。这两种微泡对正常HPDE6-C7细胞具有良好的生物相容性，对胰腺癌细胞无毒。与ALA诱导的SDT相比，在AsPC-1和BxPC-3细胞中，ALA脂质/PLGA微泡联合超声组的细胞活力有统计学意义的显著降低（<0.05）。在ALA-脂质/PLGA微泡介导的SDT中发现对凋亡率、凋亡和焦亡形态有明显影响，活性氧增强。通过研究，我们发现ALA-脂质/PLGA微泡介导的SDT是一种有前景的胰腺癌治疗方法。
Zotero 插件