Torre P M, Oliver S P
Department of Animal Science, University of Tennessee, Knoxville 37901-1071.
J Dairy Sci. 1988 Apr;71(4):1078-84. doi: 10.3168/jds.S0022-0302(88)79655-1.
Mononuclear cells were isolated from bovine blood by density gradient centrifugation to determine variation in mitogen-induced mononuclear cell activity throughout the nonlactating period. In a preliminary study, optimum concentrations of Concanavalin A, phytohemagglutinin, Salmonella typhimurium lipopolysaccharide, and three Escherichia coli lipopolysaccharides were determined using six cows as blood donors. Concanavalin A, phytohemagglutinin, and E. coli 0111:B4 lipopolysaccharide were selected for further studies. Mitogenic responses of blood mononuclear cells from five cows were evaluated at drying off, 14 to 16 and 28 to 30 d of involution, 12 to 14 d prepartum, and at parturition. Concanavalin A-treated cells exhibited greater blastogenic activity than phytohemagglutinin-treated cells. Response of cells to Concanavalin A increased slightly through 28 to 30 d of involution and decreased markedly at parturition. Blastogenic activity of cells treated with phytohemagglutinin decreased throughout the nonlactating period and was lowest at parturition. Activity of lipopolysaccharide-treated mononuclear cells increased through 28 to 30 d of involution. However, response of mononuclear cells to lipopolysaccharide was minimal compared with response to Concanavalin A and phytohemagglutinin. Variation in peripheral blood mononuclear cell activity throughout involution may parallel mammary gland mononuclear cell activity, affecting susceptibility of the mammary gland to new intramammary infections.
通过密度梯度离心法从牛血液中分离单核细胞，以确定在整个非泌乳期有丝分裂原诱导的单核细胞活性的变化。在一项初步研究中，以6头奶牛作为血液供体，确定了刀豆球蛋白A、植物血凝素、鼠伤寒沙门氏菌脂多糖和三种大肠杆菌脂多糖的最佳浓度。选择刀豆球蛋白A、植物血凝素和大肠杆菌0111:B4脂多糖进行进一步研究。对5头奶牛血液单核细胞在干奶期、 involution的14至16天和28至30天、产前12至14天以及分娩时的有丝分裂反应进行了评估。经刀豆球蛋白A处理的细胞比经植物血凝素处理的细胞表现出更大的母细胞形成活性。细胞对刀豆球蛋白A的反应在 involution的28至30天略有增加，在分娩时显著下降。经植物血凝素处理的细胞的母细胞形成活性在整个非泌乳期均下降，在分娩时最低。经脂多糖处理的单核细胞的活性在 involution的28至30天增加。然而，与对刀豆球蛋白A和植物血凝素的反应相比，单核细胞对脂多糖的反应最小。 involution期间外周血单核细胞活性的变化可能与乳腺单核细胞活性平行，影响乳腺对新的乳房内感染的易感性。
