Torre P M, Oliver S P, Konur P K
Department of Animal Science, University of Tennessee, Knoxville 37901-1071.
Cell Biol Int Rep. 1991 Mar;15(3):243-51. doi: 10.1016/0309-1651(91)90156-d.
Bovine blood and mammary gland mononuclear cells were isolated from five primiparous Holstein cows at 14-18 and 28-32 days of involution and 7-13 days prior to parturition and used in a bioassay to determine if bovine mammary mononuclear cells produced interleukin-2 in culture. Interleukin-2 production by mononuclear cells was stimulated in 24 hr cultures using concanavalin A. Bovine interleukin-2 dependent cytotoxic T-lymphocytes were used as target cells. Interleukin-2 production by mammary gland and blood mononuclear cells was comparable. Interleukin-2 production by mammary gland mononuclear cells did not vary significantly over the three time periods evaluated. However, blood mononuclear cells isolated at 28-32 days of involution produced significantly greater amounts of interleukin-2 compared to blood mononuclear cells isolated during the early nonlactating period and the prepartum period. These data suggest that bovine mononuclear cell hyporesponsiveness to mitogens during the nonlactating period is not due to a deficiency in interleukin-2 production.
从5头初产荷斯坦奶牛中分离出牛血和乳腺单核细胞，分别在 involution 的14 - 18天和28 - 32天以及分娩前7 - 13天进行分离，并用于生物测定，以确定牛乳腺单核细胞在培养中是否产生白细胞介素 - 2。在24小时培养中使用伴刀豆球蛋白A刺激单核细胞产生白细胞介素 - 2。将牛白细胞介素 - 2依赖性细胞毒性T淋巴细胞用作靶细胞。乳腺和血液单核细胞产生白细胞介素 - 2的情况相当。在所评估的三个时间段内，乳腺单核细胞产生白细胞介素 - 2的情况没有显著变化。然而，与在非泌乳早期和产前阶段分离的血液单核细胞相比，在 involution 的28 - 32天分离的血液单核细胞产生的白细胞介素 - 2量显著更多。这些数据表明，非泌乳期牛单核细胞对有丝分裂原的低反应性并非由于白细胞介素 - 2产生不足。
