Ghadiriasli Rahil, Mahmoud Mohamed A A, Wagenstaller Maria, van de Kuilen Jan-Willem, Buettner Andrea
Chair of Aroma and Smell Research, Friedrich-Alexander-Universität Erlangen-Nürnberg, Emil Fischer Center, Department of Chemistry and Pharmacy, Henkestraße 9, 91054, Erlangen, Germany; Department Sensory Analytics, Fraunhofer Institute for Process Engineering and Packaging IVV, Giggenhauser Straße 35, 85354, Freising, Germany.
Department Sensory Analytics, Fraunhofer Institute for Process Engineering and Packaging IVV, Giggenhauser Straße 35, 85354, Freising, Germany; Agricultural Biochemistry Department, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shobra, 11241, Cairo, Egypt.
Talanta. 2020 Dec 1;220:121380. doi: 10.1016/j.talanta.2020.121380. Epub 2020 Jul 13.
The wood of the Cembran pine (Pinus cembra L.), also known as Swiss pine, Arolla pine or Stone pine, has a prominent and long-lasting odor which has been reported to relieve stress in humans. However, the odorants responsible for this are unknown and it is also unclear whether the odor profile depends on the origin of the trees, namely different geographical regions and pedoclimatic conditions. Therefore, in this study human sensory analyses together with isolation and analysis of the target odorants via solvent-assisted flavor evaporation were performed. Afterwards, comparative odor extract dilution analysis (cOEDA) was applied to investigate the odorous constituents of pinewood samples from forests in Austria and Italy. Specific odorants were thereby identified using one-dimensional and two-dimensional gas chromatography-mass spectrometry/olfactometry using two capillary columns with different polarities (DB-5 and DB-FFAP). In total, 103 odorants were detected with successful identification of 98 of these substances. The identified odorants were predominately monoterpenes and sesquiterpenes as well as organic acids. Several compounds including germacrene D, thymol, carvacrol, rotundone, β-caryophyllene, α-humulene, cinnamaldehyde, and ethyl cinnamate are reported here for the first time as odorous constituents of pinewood. Principal component analysis of the data obtained from sensory evaluation and cOEDA indicated that terpenes and sesquiterpenes were correlated with higher hedonic rates for the wood samples. Moreover, a Mantel test between the geographical distances of the trees and their sensory and cOEDA values demonstrated that the origin of the trees had a significant impact on the sensory characteristics of the wood, in agreement with the differences in the relative proportions of different volatile components. As such, the odor of Cembran pinewood is prone to variation but, yet, unique.
西伯利亚刺柏（Pinus cembra L.），也被称为瑞士松、阿勒拉松或石松，其木材具有显著且持久的气味，据报道这种气味能缓解人类压力。然而，产生这种气味的气味物质尚不清楚，而且气味特征是否取决于树木的来源，即不同的地理区域和土壤气候条件也不明确。因此，在本研究中，进行了人类感官分析，并通过溶剂辅助风味蒸发对目标气味物质进行了分离和分析。之后，应用比较气味提取物稀释分析（cOEDA）来研究来自奥地利和意大利森林的松木样本的气味成分。通过使用两根不同极性的毛细管柱（DB - 5和DB - FFAP）的一维和二维气相色谱 - 质谱联用/嗅觉测量法，从而鉴定出了特定的气味物质。总共检测到103种气味物质，其中98种被成功鉴定。鉴定出的气味物质主要是单萜类、倍半萜类以及有机酸。包括杜松烯D、百里香酚、香芹酚、圆柚酮、β - 石竹烯、α - 葎草烯、肉桂醛和肉桂酸乙酯在内的几种化合物，在此首次被报道为松木的气味成分。对感官评价和cOEDA获得的数据进行主成分分析表明，萜类和倍半萜类与木材样本较高的愉悦度相关。此外，树木地理距离与其感官和cOEDA值之间的曼特尔检验表明，树木的来源对木材的感官特征有显著影响，这与不同挥发性成分相对比例的差异一致。因此，西伯利亚刺柏木材的气味容易变化，但却独具特色。
