Şahin Samet, Caglayan Mustafa Oguzhan, Üstündağ Zafer
Bilecik Şeyh Edebali University, Department of Bioengineering, 11230, Bilecik, Turkey.
Bilecik Şeyh Edebali University, Department of Bioengineering, 11230, Bilecik, Turkey.
Talanta. 2020 Dec 1;220:121437. doi: 10.1016/j.talanta.2020.121437. Epub 2020 Jul 24.
Heavy metal ion pollution is a severe problem in environmental protection and especially in human health due to their bioaccumulation in organisms. Mercury (II) (Hg), even at low concentrations, can lead to DNA damage and give permanent harm to the central nervous system by easily passing through biological membranes. Therefore, sensitive detection and monitoring of Hg is of particular interest with significant specificity. In this review, aptamer-based strategies in combination with nanostructures as well as several other strategies to solve addressed problems in sensor development for Hg are discussed in detail. In particular, the analytical performance of different aptamer and oligonucleotide-based strategies using different signal improvement approaches based on nanoparticles were compared within each strategy and in between. Although quite a number of the suggested methodologies analyzed in this review fulfills the standard requirements, further development is still needed on real sample analysis and analytical performance parameters.
重金属离子污染是环境保护尤其是人类健康领域的一个严峻问题，因为它们会在生物体内生物累积。汞（II）（Hg）即使浓度很低，也会导致DNA损伤，并通过轻易穿透生物膜对中枢神经系统造成永久性损害。因此，对汞进行灵敏检测和监测具有特别重要的意义，且需要具有显著的特异性。在这篇综述中，详细讨论了基于适配体的策略与纳米结构相结合的方法，以及其他几种解决汞传感器开发中相关问题的策略。特别是，在每种策略内部以及不同策略之间，比较了使用基于纳米颗粒的不同信号增强方法的不同基于适配体和寡核苷酸的策略的分析性能。尽管本综述中分析的许多建议方法都符合标准要求，但在实际样品分析和分析性能参数方面仍需要进一步发展。
